Ataxin 1 Antibody (YA4739)

Cat. No.: HY-P85047: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Ataxin 1 Antibody (YA4739) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Ataxin 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Ataxin 1 Antibody (YA4739) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Ataxin 1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 87 kDa;

Species Reactivity

Human

SwissProt ID

P54253

Gene ID

6310 [NCBI]

Immunogen

Purified recombinant fragment of human Ataxin-1 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism (PubMed:21475249). In concert with CIC and ATXN1L, involved in brain development (By similarity)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Widely distributed throughout the body

Induction:ATXN1 protein levels are directly regulated by PUM1 protein: PUM1 acts by binding to the 3'-UTR of ATXN1 mRNA, affecting ATXN1 mRNA stability and leading to reduced ATXN1 protein levels

Isoforms & Post-Translational Modification：P54253 has 1 isomers: P54253-1: 86923 Da (predicted).
Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity;Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract;Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract

Subunit：Homooligomer (PubMed:9097953). Interacts with CIC (By similarity). Interacts with ANP32A, PQBP1, UBQLN4, ATXN1L and USP7 (PubMed:11001934, PubMed:12062018, PubMed:12093161, PubMed:16121196, PubMed:9353121). Directly interacts with RBPJ; this interaction is disrupted in the presence of Notch intracellular domain. Competes with ATXN1L for RBPJ-binding (PubMed:21475249). Found in a complex with CIC and ATXN1L (By similarity)

Synonyms

ATXN1; ATX1; SCA1; Ataxin-1; Spinocerebellar ataxia type 1 protein

Documentation

User Guide for Antibodies (1077 KB)