Cytokeratin 17 Antibody (YA3438)

Cat. No.: HY-P83702: Fiche technique COA
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Cytokeratin 17 Antibody (YA3438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 17.

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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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Cytokeratin 17 Antibody (YA3438) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

Cytokeratin 17 Antibody (YA3438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 17.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Masse moléculaire

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q04695

Gene ID

3872 [NCBI]

Immunogen

Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:1500

Sensitivity	Endogenous	Pureté	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from A431 (lane 2(20μg) , A431(lane 3(40μg),using Cytokeratin 17 Antibody (HY-P83702). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 17 with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of SK-BR-3 cells labeling Cytokeratin 17 with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:This protein is expressed in the outer root sheath and medulla of hair follicles (especially in eyebrows and beards) , fingertips, nail matrix and nail bed epithelium, stratified squamous epithelium of the mucosa, basal cells of the oral epithelium, palmar and plantar epithelium, sweat glands, and mammary glands. Furthermore, it is also expressed in the myoepithelial cells of the prostate, basal layer of the bladder, sebaceous gland cambium cells, and the adventitia of the cervix (protein level) .

Induction:Induced in damaged or stressed epidermis. Induced by the cytokines interferon-gamma (IFN-gamma) , tumor necrosis factor alpha (TNF-alpha) and transforming growth factor-alpha (TGF-alpha) , and by the potent NF-kappa B inhibitor compounds Bay 11-7082 and Bay 11-7085. Down-regulated by the drug Imatinib

Positive sample: A431 cell lysates, Mouse skin tissue lysate, Rat skin tissue lysate, SiHa, human prostate tissue, human skin tissue, human tonsil tissue, mouse prostate tissue, human cervical

Subunit：Heterodimer of a type I and a type II keratin. KRT17 associates with KRT6 isomers (KRT6A or KRT6B).

RRID

AB_3676585

Database

Entrez Gene: 3872 Human ; 16667 Mouse ; 287702 Rat

SwissProt: Q04695 Human ; Q9QWL7 Mouse ; Q6IFU8 Rat

OMIM: 167210 Human

Synonyms

39.1 antibody; CK 17 antibody; CK17 antibody; CK-17 antibody; Cytokeratin-17 antibody; K17 antibody; K1C17_HUMAN antibody; Keratin 17 antibody; keratin 17 epitope S1 antibody; keratin 17 epitope S2 antibody; keratin 17 epitope S4 antibody; Keratin 17, type I antibody; Keratin antibody; Keratin type I cytoskeletal 17 antibody; keratin, type i cytoskeletal 17 [version 1] antibody; Keratin-17 antibody; KRT17 antibody; PC antibody; PC2 antibody; PCHC1 antibody; type I cytoskeletal 17 antibody;

Documentation

Select Batch:

Fiche technique (235 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cytokeratin 17 Antibody (YA3438) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytokeratin 17 Antibody (YA3438)

Cytokeratin 17 Antibody (YA3438) Recommandations en vedette:

Product Detail

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Background

Description

Cytokeratin 17 Antibody (YA3438) Related Classifications

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