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T Box Transcription Factor T Antibody (YA3523)

(Synonyms: TFT,TBXT)
Customer Review

Based on 1 Customer Validation

T Box Transcription Factor T Antibody (YA3523) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to T Box Transcription Factor T.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • T Box Transcription Factor T Antibody (YA3523) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

T Box Transcription Factor T Antibody (YA3523) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to T Box Transcription Factor T.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47/41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

Purified recombinant fragment of human T (AA: 257-309 ) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718776

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    T Box Transcription Factor T is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It was detected in the testes but not in other normal tissues. It was also detected in lung tumors (protein levels) .

  • Isoforms & Post-Translational Modification

    O15178 has 2 isomers: O15178-1: 47443 Da (predicted); O15178-2: 41078 Da (predicted).

  • Subunit

    Monomer

  • SwissProt ID

    O15178

  • Gene ID
    6862 [NCBI]
  • Synonyms

    TFT,TBXT

T Box Transcription Factor T Antibody (YA3523) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    1/16
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • T Box Transcription Factor T Antibody (YA3523) Product Image
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • Experimental Validation Results for T Box Transcription Factor T Antibody (YA3523)
  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded SW948 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded OE-19 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded RT-112 syngeneic tumor tissue using T Box Transcription Factor T antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P83826, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P
  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P
  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P
  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P
  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P
  • T Box Transcription Factor T Antibody (YA3523) Product Image IHC-P