Hepatic Lipase Antibody
(Synonyms: HTGL, LIPC, Hepatic triacylglycerol lipase, HL, Hepatic lipase, Lipase member C, Lysophospholipase, Phospholipase A1)Hepatic Lipase Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Hepatic Lipase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
Hepatic Lipase Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Hepatic Lipase.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
Synthetic peptide corresponding to the center region of human Hepatic Lipase.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Hepatic Lipase catalyzes the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins, including chylomicrons, intermediate density lipoproteins (IDL), low density lipoproteins (LDL) of large size and high density lipoproteins (HDL), releasing free fatty acids (FFA) and smaller lipoprotein particles. Also exhibits lysophospholipase activity (By similarity). Can hydrolyze both neutral lipid and phospholipid substrates but shows a greater binding affinity for neutral lipid substrates than phospholipid substrates (By similarity). In native LDL, preferentially hydrolyzes the phosphatidylcholine species containing polyunsaturated fatty acids at sn-2 position[1][2][3][4].
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Subcellular Localization
Secreted
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Isoforms & Post-Translational Modification
Hepatic Lipase has an amino acid length of 499, molecular weight is 55914 Da.
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Subunit
Homodimer
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SwissProt ID
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Synonyms
HTGL, LIPC, Hepatic triacylglycerol lipase, HL, Hepatic lipase, Lipase member C, Lysophospholipase, Phospholipase A1
Documentation
References
[1]. McCoy MG, et al. Characterization of the lipolytic activity of endothelial lipase. J Lipid Res. 2002 Jun;43(6):921-9. [Content Brief]
[2]. Yang P, et al. Regulation of hepatic lipase activity by sphingomyelin in plasma lipoproteins. Biochim Biophys Acta. 2015 Oct;1851(10):1327-36. [Content Brief]
[3]. Dugi KA, et al. Human hepatic and lipoprotein lipase: the loop covering the catalytic site mediates lipase substrate specificity. J Biol Chem. 1995 Oct 27;270(43):25396-401. [Content Brief]
[4]. Hill JS, et al. Human hepatic lipase subunit structure determination. J Biol Chem. 1996 Sep 13;271(37):22931-6. [Content Brief]
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