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Cathepsin L Antibody (YA6531)

(Synonyms: Cathepsin L antibody; cathepsin L, 1 b antibody; Cathepsin L1 antibody; Cathepsin L1 light chain antibody; CathepsinL antibody; CATL antibody; CATL1_HUMAN antibody; cb15 antibody; CTSL antibody; CTSL1 antibody; Cathepsin L antibody; cathepsin L, 1 b antibody; Cathepsin L1 antibody; Cathepsin L1 light chain antibody; CathepsinL antibody; CATL antibody; CATL1_HUMAN antibody; cb15 antibody; CTSL antibody; CTSL1 antibody; ctsl1b antibody; FLJ31037 antibody; hgg1 antibody; Major excreted protein antibody; MEP antibody; MGC123162 antibody; wu:fb30g09 antibody; )
Customer Review

Based on 1 Customer Validation

Cathepsin L Antibody (YA6531) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin L.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Cathepsin L Antibody (YA6531) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:5000 1:5000 1:1000

Product Details

Description

Cathepsin L Antibody (YA6531) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cathepsin L.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 20-45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

Recombinant protein within human Cathepsin L aa 84-333 / 333.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Western blot analysis of extracts from HCT116 (lane 2(20μg), A549 (lane 3(20μg), HepG2 (lane 4(20μg), HT-29 (lane 5(20μg) using Cathepsin L Antibody（HY-P86838). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine proteinase involved in lysosomal proteolysis, MHC class II antigen presentation, prohormone processing, and extracellular matrix remodeling[1]. Mechanistically, CTSL supports invariant chain degradation in cortical thymic epithelial cells and contributes to CD4+ T-cell selection in the thymus[2]. It also regulates positively selecting peptide ligands independently of invariant chain degradation, showing that CTSL affects thymic MHC class II presentation through more than one mechanism[3]. In skin models, CTSL deficiency causes periodic hair loss, epidermal hyperplasia, altered hair follicle morphogenesis, and disturbed hair follicle cycling[1]. Keratinocyte hyperproliferation in CTSL-knockout mice results from enhanced recycling of growth factors and receptors from endosomes to the plasma membrane[4]. Compared with related isoforms, human cathepsin V is highly homologous to cathepsin L, is expressed in human thymus and testis, and lacks a mouse orthologue[5]. In SARS-CoV-2 models, CTSL cleaves spike protein and enhances viral entry, while CTSL inhibition reduces pseudovirus infection in vitro and in vivo[6]. For experimental applications, small-molecule inhibitors and functional probes help define human CTSL mechanisms in specific cellular contexts[7].

  • Subcellular Localization

    Lysosome,Apical cell membrane,Cytoplasmic vesicle, secretory vesicle, chromaffin granule,Secreted, extracellular space,Secreted,Nucleus

  • Isoforms & Post-Translational Modification

    P07711 has two isomers: P07711-1: 37564 Da (predicted); P07711-3: 31173 Da (predicted).
    During export along the endocytic pathway, pro-CTSL undergoes several proteolytic cleavages to generate the CTSL single-chain and two-chain mature forms, composed of a heavy chain linked to a light chain by disulfide bonds (By similarity)

  • Subunit

    Dimer of a heavy and a light chain linked by disulfide bonds

  • SwissProt ID

    P07711

  • Gene ID
    1514 [NCBI]
  • Synonyms

    Cathepsin L antibody; cathepsin L, 1 b antibody; Cathepsin L1 antibody; Cathepsin L1 light chain antibody; CathepsinL antibody; CATL antibody; CATL1_HUMAN antibody; cb15 antibody; CTSL antibody; CTSL1 antibody; Cathepsin L antibody; cathepsin L, 1 b antibody; Cathepsin L1 antibody; Cathepsin L1 light chain antibody; CathepsinL antibody; CATL antibody; CATL1_HUMAN antibody; cb15 antibody; CTSL antibody; CTSL1 antibody; ctsl1b antibody; FLJ31037 antibody; hgg1 antibody; Major excreted protein antibody; MEP antibody; MGC123162 antibody; wu:fb30g09 antibody;

References

[1]. Roth W, et al. Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling. FASEB J. 2000 Oct;14(13):2075-86. [Content Brief]

[2]. Nakagawa T, et al. Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus. Science. 1998 Apr 17;280(5362):450-3. [Content Brief]

[3]. Honey K, et al. Cathepsin L regulates CD4+ T cell selection independently of its effect on invariant chain: a role in the generation of positively selecting peptide ligands. J Exp Med. 2002 May 20;195(10):1349-58. [Content Brief]

[4]. Reinheckel T, et al. The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling. J Cell Sci. 2005 Aug 1;118(Pt 15):3387-95. [Content Brief]

[5]. Tolosa E, et al. Cathepsin V is involved in the degradation of invariant chain in human thymus and is overexpressed in myasthenia gravis. J Clin Invest. 2003 Aug;112(4):517-26. [Content Brief]

[6]. Zhao MM, et al. Cathepsin L plays a key role in SARS-CoV-2 infection in humans and humanized mice and is a promising target for new drug development. Signal Transduct Target Ther. 2021 Mar 27;6(1):134. [Content Brief]

[7]. Dana D, et al. A Review of Small Molecule Inhibitors and Functional Probes of Human Cathepsin L. Molecules. 2020 Feb 6;25(3):698. [Content Brief]

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Cathepsin L Antibody (YA6531) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane 2(20μg), A549 (lane 3(20μg), HepG2 (lane 4(20μg), HT-29 (lane 5(20μg) using Cathepsin L Antibody（HY-P86838). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    1/16
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Cathepsin L Antibody (YA6531) Product Image
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane 2(20μg), A549 (lane 3(20μg), HepG2 (lane 4(20μg), HT-29 (lane 5(20μg) using Cathepsin L Antibody（HY-P86838). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Experimental Validation Results for Cathepsin L Antibody (YA6531)
  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116 (lane 2(20μg), A549 (lane 3(20μg), HepG2 (lane 4(20μg), HT-29 (lane 5(20μg) using Cathepsin L Antibody（HY-P86838). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Cathepsin L Antibody (HY-P86838, 1/5000) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 7℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cathepsin L Antibody (YA6531) Product Image WB
  • Cathepsin L Antibody (YA6531) Product Image IHC-P
  • Cathepsin L Antibody (YA6531) Product Image IHC-P
  • Cathepsin L Antibody (YA6531) Product Image IHC-P
  • Cathepsin L Antibody (YA6531) Product Image IHC-P
  • Cathepsin L Antibody (YA6531) Product Image IHC-P
  • Cathepsin L Antibody (YA6531) Product Image IHC-P