BUBR1 Antibody (YA2757)
(Synonyms: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1)
Based on 1 Customer Validation
BUBR1 Antibody (YA2757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUBR1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:200-1:500 | 1:20-1:50 |
Product Details
BUBR1 Antibody (YA2757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUBR1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 120 kDa
A synthetic peptide of human BubR1 aa48-237.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BUBR1 is an essential spindle assembly checkpoint protein that prevents premature anaphase onset and supports chromosome alignment and kinetochore-microtubule attachment[1]. Mechanistically, BUBR1 inhibits APC/C^Cdc20 through its N-terminal Cdc20-binding KEN-box region, allowing cyclin B accumulation before mitotic entry[2]. At kinetochores, BUBR1 links mitotic checkpoint signaling with chromosome-spindle attachment control and regulates CENP-A/Aurora kinase-associated attachment stability[3]. In human cells, BUBR1/BUB3 exists in functionally distinct kinetochore pools: a Bub1-dependent pool supports chromosome alignment, whereas KNL1-MELT binding strengthens checkpoint-complex incorporation[4]. Compared with Bub1, Bub1 is required for kinetochore localization of BUBR1, CENP-E, CENP-F, and Mad2, while BUBR1 is not required for kinetochore localization of Bub1 in human somatic cells[5]. In disease models, reduced BUBR1 causes progressive aneuploidy, senescence, infertility, and progeroid phenotypes in mice, whereas sustained high-level BUBR1 expression preserves genomic integrity and reduces tumorigenesis[6][7]. For experimental applications, bubristatin acts as a selective BUBR1 kinase antagonist and helps dissect BUBR1-CENP-E control of end-on microtubule capture[8].
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Subcellular Localization
Cytoplasm; Nucleus; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_specificity:Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index
Induction:Induced during mitosis -
Isoforms & Post-Translational Modification
O60566 has 3 isomers: O60566-1: 119545 Da (predicted); O60566-2: 105890 Da (predicted); O60566-3: 121386 Da (predicted).
Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610;Acetylation at Lys-250 regulates its degradation and timing in anaphase entry;Ubiquitinated. Degraded by the proteasome (PubMed:19407811). Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex (PubMed:35217622);Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore;Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase -
Subunit
Interacts with CENPE (PubMed:12925705, PubMed:19625775). Interacts with PLK1 (PubMed:16760428, PubMed:17376779, PubMed:17785528, PubMed:19503101). Part of a complex containing BUB3, CDC20 and BUB1B (PubMed:11702782). Interacts with anaphase-promoting complex/cyclosome (APC/C) (PubMed:10477750). Interacts with KNL1 (PubMed:17981135). Interacts with KAT2B (PubMed:19407811). Interacts with RIPK3 (PubMed:29883609). Interacts with the closed conformation form of MAD2L1 (PubMed:29162720)
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SwissProt ID
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Synonyms
BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
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Research Field
Cell Biology
Documentation
References
[1]. Bloom CR, et al. Physiological relevance of post-translational regulation of the spindle assembly checkpoint protein BubR1. Cell Biosci. 2021 Apr 23;11(1):76. [Content Brief]
[2]. Malureanu LA, et al. BubR1 N terminus acts as a soluble inhibitor of cyclin B degradation by APC/C(Cdc20) in interphase. Dev Cell. 2009 Jan;16(1):118-31. [Content Brief]
[3]. Lampson MA, et al. The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments. Nat Cell Biol. 2005 Jan;7(1):93-8. [Content Brief]
[4]. Zhang G, et al. Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. Nat Commun. 2016 Jul 26;7:12256. [Content Brief]
[5]. Johnson VL, et al. Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression. J Cell Sci. 2004 Mar 15;117(Pt 8):1577-89. [Content Brief]
[6]. Baker DJ, et al. BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat Genet. 2004 Jul;36(7):744-9. [Content Brief]
[7]. Baker DJ, et al. Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan. Nat Cell Biol. 2013 Jan;15(1):96-102. [Content Brief]
[8]. Huang Y, et al. BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules. Cell Res. 2019 Jul;29(7):562-578. [Content Brief]
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