GLI-1 Antibody (YA7555)
GLI-1 Antibody (YA7555) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GLI-1.
-
Host:
Rabbit
-
Isotype:
IgG, Kappa
-
Application:
WB, ICC/IF, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
GLI-1 Antibody (YA7555) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GLI-1.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 160 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 118 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG, Kappa
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
GLI-1 acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling |Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration[1][2][3][4][5][6][7][8][9][10].
-
Subcellular Localization
Cytoplasm,Nucleus,Cytoplasm,Nucleus
-
Expression
Tissue_Specificity: Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)
Induction: Isoform 1 and isoform 2 are amplified in glioblastoma cells -
Isoforms & Post-Translational Modification
P08151 has three isomers: P08151-1: 117904 Da (predicted); P08151-2: 113676 Da (predicted); P08151-3: 104627 Da (predicted).
Phosphorylated in vitro by ULK3丨Acetylation at Lys-518 down-regulates transcriptional activity丨Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity -
Subunit
Interacts with KIF7 (By similarity)
-
SwissProt ID
Documentation
Powered by Bioz
