GLI-1 Antibody (YA7555)

Cat. No.: HY-P87870: Data Sheet User Guide for Antibodies
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GLI-1 Antibody (YA7555) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GLI-1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

GLI-1 Antibody (YA7555) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GLI-1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 118 kDa;

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P08151

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a transcriptional activator (PubMed:10806483, PubMed:19706761, PubMed:19878745, PubMed:24076122, PubMed:24217340, PubMed:24311597). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:24217340, PubMed:8378770). Regulates the transcription of specific genes during normal development (PubMed:19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407)|Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration

Subcellular Localization：Cytoplasm,Nucleus,Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)
Induction: Isoform 1 and isoform 2 are amplified in glioblastoma cells

Isoforms & Post-Translational Modification：P08151 has three isomers: P08151-1: 117904 Da (predicted); P08151-2: 113676 Da (predicted); P08151-3: 104627 Da (predicted).
Phosphorylated in vitro by ULK3丨Acetylation at Lys-518 down-regulates transcriptional activity丨Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity

Subunit：Interacts with KIF7 (By similarity)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mv4-11-cell.html [Content Brief]