RIOK3 Antibody
(Synonyms: SUDD, RIOK3, Serine/threonine-protein kinase RIO3, RIO kinase 3, sudD homolog)RIOK3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RIOK3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
RIOK3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RIOK3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
Synthetic peptide corresponding to the center region of human RIOK3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RIOK3 is a Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappa-B signaling pathway[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm, cytosol
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Expression
Tissue_Specificity: Widely expressed. -
Isoforms & Post-Translational Modification
RIOK3 has 2 isoforms, O14730-1: amino acid length is 519, molecular weight is 59093 Da (predicted); O14730-2: amino acid length is 516, molecular weight is 58671 Da (predicted).Autophosphorylated (in vitro)
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Subunit
Interacts with CASP10.
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SwissProt ID
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Synonyms
SUDD, RIOK3, Serine/threonine-protein kinase RIO3, RIO kinase 3, sudD homolog
Documentation
[1]. Coria AR, et al. The integrated stress response regulates 18S nonfunctional rRNA decay in mammals. Mol Cell. 2025 Feb 20;85(4):787-801.e8. [Content Brief]
[2]. Huang Z, et al. RIOK3 mediates the degradation of 40S ribosomes. Mol Cell. 2025 Feb 20;85(4):802-814.e12. [Content Brief]
[3]. Ford PW, et al. RNF10 and RIOK3 facilitate 40S ribosomal subunit degradation upon 60S biogenesis disruption or amino acid starvation. Cell Rep. 2025 Mar 25;44(3):115371. [Content Brief]
[4]. Feng J, et al. RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. J Virol. 2014 Jul;88(14):7987-97. [Content Brief]
[5]. Takashima K, et al. RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell Rep. 2015 Apr 14;11(2):192-200. [Content Brief]
[6]. Shan J, et al. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Mol Cell Biochem. 2009 Dec;332(1-2):113-20. [Content Brief]
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