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Ly6A/E Antibody (YA2518)

(Synonyms: Ly-6A.2/Ly-6E.1; Ly6a; Ly6al; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen Ly 6A.2/Ly 6E.1; SCA-1; Stem cell antigen 1; T cell activating protein; TAP)
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Based on 1 Customer Validation

Ly6A/E Antibody (YA2518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6A/E.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ly6A/E Antibody (YA2518)
  • Ly6A/E Antibody (YA2518) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Ly6A/E Antibody (YA2518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6A/E.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Species Reactivity Database

Entrez Gene: 110454 Mouse

SwissProt: P05533 Mouse

Immunogen

A synthesized peptide derived from mouse LY6A aa50-80.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104869

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ly6A/E Antibody (YA2518)
    Western blot analysis of extracts from C2C12 (lane 2(20μg)) and Mouse kidney (lane 3(20μg)) using Ly6A/E Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Ly6A/E is a T-cell activation

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Expression


    Tissue_specificity:Widely expressed

  • SwissProt ID

    P05533

  • Gene ID
    110454 [NCBI]
  • Synonyms

    Ly-6A.2/Ly-6E.1; Ly6a; Ly6al; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen Ly 6A.2/Ly 6E.1; SCA-1; Stem cell antigen 1; T cell activating protein; TAP

  • Research Field

    Immunology

Ly6A/E Antibody (YA2518) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C2C12 (lane 2(20μg)) and Mouse kidney (lane 3(20μg)) using Ly6A/E Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ly6A/E Antibody (YA2518) Product Image
    1/16
  • Experimental Validation Results for Ly6A/E Antibody (YA2518)
  • Ly6A/E Antibody (YA2518) Product Image
  • Experimental Validation Results for Ly6A/E Antibody (YA2518)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C2C12 (lane 2(20μg)) and Mouse kidney (lane 3(20μg)) using Ly6A/E Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ly6A/E Antibody (YA2518)
  • Ly6A/E Antibody (YA2518) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C2C12 (lane 2(20μg)) and Mouse kidney (lane 3(20μg)) using Ly6A/E Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ly6A/E Antibody (YA2518) Product Image WB