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GPX4 Antibody

(Synonyms: Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4)
5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

GPX4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GPX4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • GPX4 Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

GPX4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GPX4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human GPX4 aa18-65.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102669

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GPX4 Antibody
    Western blot analysis of extracts from HEK293(lane 2(20μg) , A549(lane 3(20ug) and Hepg2(lane 4(20ug)using Glutathione Peroxidase 4 Antibody (HY-P80692) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GPX4 Antibody
    Immunocytochemistry analysis of C6 cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPX4 Antibody
    Immunocytochemistry analysis of HELA cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPX4 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GPX4 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    GPX4 (glutathione peroxidase 4) is a selenium-dependent phospholipid hydroperoxidase that maintains cellular redox homeostasis by reducing complex lipid hydroperoxides to their corresponding lipid alcohols using glutathione as a cofactor, thereby preventing membrane lipid peroxidation and oxidative damage[1][2][3]. Mechanistically, GPX4 functions as the central regulator and major endogenous suppressor of ferroptosis, an iron-dependent form of regulated cell death driven by the accumulation of phospholipid hydroperoxides, and loss of GPX4 activity promotes lipid peroxide accumulation and ferroptotic cell death[2][4][5][6]. Through this lipid peroxide detoxification pathway, GPX4 contributes to the maintenance of cellular viability, immune homeostasis, and tissue integrity, while impaired GPX4 function has been associated with cancer, neurodegenerative disorders, inflammation, ischemia-reperfusion injury, and other ferroptosis-related pathologies[1][7][8]. Compared with other glutathione peroxidase family members, GPX4 possesses a unique ability to directly reduce phospholipid hydroperoxides and oxidized complex lipids, distinguishing it as a specialized phospholipid repair enzyme and a master regulator of ferroptosis[2][9][10]. Distinct cytosolic, mitochondrial, and nuclear GPX4 isoforms have been described, with cytosolic GPX4 identified as essential for embryonic development and cell survival[7][8]. For experimental applications, pharmacological GPX4 inhibitors such as RSL3 and ML162 are widely used to induce ferroptosis, enabling mechanistic studies of lipid peroxidation, redox regulation, cancer vulnerability, and ferroptosis-targeted therapeutic strategies[11][12].

  • Subcellular Localization

    Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:Present primarily in testis. Expressed in platelets (at protein level).

  • Isoforms & Post-Translational Modification

    P36969 has 2 isomers: P36969-1: 22175 Da (predicted); P36969-2: 19525 Da (predicted).
    Degraded via chaperone-mediated autophagy, promoting ferroptosis. Hydroxylation by EGLN3/PHD3 promotes its stabilization by preventing degradation via chaperone-mediated autophagy.

  • Subunit

    Monomer. Has a tendency to form higher mass oligomers (PubMed:17630701). Interacts with FUNDC1; this interaction promotes GPX4 recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy (PubMed:36828120). Interacts (via KFERQ motif) with HSPA8; promoting its association with LAMP2 and degradation via chaperone-mediated autophagy (By similarity).

  • SwissProt ID

    P36969

  • Gene ID
    2879 [NCBI]
  • Synonyms

    Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4

  • Research Field

    Signal Transduction

References

[1]. Weaver K, et al. The Selenoprotein Glutathione Peroxidase 4: From Molecular Mechanisms to Novel Therapeutic Opportunities. Biomedicines. 2022 Apr 13;10(4):891. [Content Brief]

[2]. Ma T, et al. GPX4-independent ferroptosis-a new strategy in disease's therapy. Cell Death Discov. 2022 Oct 30;8(1):434. [Content Brief]

[3]. Xia Y, et al. SIRT1 activation ameliorates rhesus monkey liver fibrosis by inhibiting the TGF-β/smad signaling pathway. Chem Biol Interact. 2024 May 1;394:110979. [Content Brief]

[4]. Li J, et al. Ferroptosis: past, present and future. Cell Death Dis. 2020 Feb 3;11(2):88. [Content Brief]

[5]. Ursini F, et al. Lipid peroxidation and ferroptosis: The role of GSH and GPx4. Free Radic Biol Med. 2020 May 20;152:175-185. [Content Brief]

[6]. Ursini F, et al. A white paper on Phospholipid Hydroperoxide Glutathione Peroxidase (GPx4) forty years later. Free Radic Biol Med. 2022 Aug 1;188:117-133. [Content Brief]

[7]. Xie Y, et al. GPX4 in cell death, autophagy, and disease. Autophagy. 2023 Oct;19(10):2621-2638. [Content Brief]

[8]. GPX4 gene information from NCBI.

[9]. Liu Y, et al. GPX4: The hub of lipid oxidation, ferroptosis, disease and treatment. Biochim Biophys Acta Rev Cancer. 2023 May;1878(3):188890. [Content Brief]

[10]. Sciencedirect.topics.

[11]. Cui C, et al. Post-Translational Modification of GPX4 is a Promising Target for Treating Ferroptosis-Related Diseases. Front Mol Biosci. 2022 May 12;9:901565. [Content Brief]

[12]. Sekhar KR, et al. Glutathione peroxidase 4 inhibition induces ferroptosis and mTOR pathway suppression in thyroid cancer. Sci Rep. 2022 Nov 12;12(1):19396. [Content Brief]

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GPX4 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg) , A549(lane 3(20ug) and Hepg2(lane 4(20ug)using Glutathione Peroxidase 4 Antibody (HY-P80692) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GPX4 Antibody Product Image
    1/16
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • GPX4 Antibody Product Image
  • Experimental Validation Results for GPX4 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg) , A549(lane 3(20ug) and Hepg2(lane 4(20ug)using Glutathione Peroxidase 4 Antibody (HY-P80692) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GPX4 Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPX4 Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPX4 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GPX4 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • Experimental Validation Results for GPX4 Antibody
  • GPX4 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293(lane 2(20μg) , A549(lane 3(20ug) and Hepg2(lane 4(20ug)using Glutathione Peroxidase 4 Antibody (HY-P80692) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GPX4 Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GPX4 Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HELA cells labeling Glutathione Peroxidase 4 with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glutathione Peroxidase 4 Antibody (HY-P80692) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GPX4 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GPX4 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Glutathione Peroxidase 4 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GPX4 Antibody Product Image WB
  • GPX4 Antibody Product Image ICC
  • GPX4 Antibody Product Image ICC
  • GPX4 Antibody Product Image IHC-P
  • GPX4 Antibody Product Image IHC-P