Ferritin Heavy Chain Antibody (YA443)

Cat. No.: HY-P80670: Data Sheet COA
SDS
User Guide for Antibodies
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Ferritin Heavy Chain Antibody (YA443) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ferritin Heavy Chain.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Ferritin Heavy Chain Antibody (YA443) Featured Recommendations:

9 Publications Citing Use of MCE Ferritin Heavy Chain Antibody (YA443)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Ferritin Heavy Chain Antibody (YA443) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ferritin Heavy Chain.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 21 kDa;

Observed band size: 21 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

P02794

Gene ID

2495 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Ferritin.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB ICC

Western blot analysis of extracts from C6 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Ferritin Heavy Chain (HY-P80670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293)

Subcellular Localization：Cytoplasm; Lysosome; Cytoplasmic vesicle, autophagosome

Expression：
Tissue_specificity:Expression in the liver

Subunit：Oligomer of 24 subunits (PubMed:9159481). There are two types of subunits: L (light) chain and H (heavy) chain (PubMed:9159481). The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant (PubMed:9159481). The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited (PubMed:9159481). Interacts with NCOA4; NCOA4 promotes targeting of the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:24695223)

RRID

AB_3102656

Database

Entrez Gene: 2495 Human ; 14319 Mouse ; 25319 Rat

SwissProt: P02794 Human ; P09528 Mouse ; P19132 Rat

OMIM: 615517 Human

Research Field

Neuroscience

Synonyms

FTH1; FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin heavy chain; Ferritin H subunit; Cell proliferation-inducing gene 15 protein

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Ferritin Heavy Chain Antibody (YA443) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ferritin Heavy Chain Antibody (YA443)

Ferritin Heavy Chain Antibody (YA443) Featured Recommendations:

9 Publications Citing Use of MCE Ferritin Heavy Chain Antibody (YA443)

Product Detail

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Background

Documentation

Ferritin Heavy Chain Antibody (YA443) Related Classifications

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