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Ferritin Heavy Chain Antibody (YA443)

(Synonyms: FTH1; FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin heavy chain; Ferritin H subunit; Cell proliferation-inducing gene 15 protein)
10 Cited Publications
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Based on 10 publication(s) in Google Scholar

Ferritin Heavy Chain Antibody (YA443) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ferritin Heavy Chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat, Hamster

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Ferritin Heavy Chain Antibody (YA443) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Ferritin Heavy Chain Antibody (YA443) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ferritin Heavy Chain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Hamster
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Ferritin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102656

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    Western blot analysis of extracts from C6 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Ferritin Heavy Chain (HY-P80670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Ferritin Heavy Chain is a Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron[1][2][3].

  • Subcellular Localization

    Cytoplasm; Lysosome; Cytoplasmic vesicle, autophagosome

  • Expression


    Tissue_specificity:Expression in the liver

  • Subunit

    Oligomer of 24 subunits (PubMed:9159481). There are two types of subunits: L (light) chain and H (heavy) chain (PubMed:9159481). The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant (PubMed:9159481). The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited (PubMed:9159481). Interacts with NCOA4; NCOA4 promotes targeting of the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:24695223)

  • SwissProt ID

    P02794

  • Gene ID
    2495 [NCBI]
  • Synonyms

    FTH1; FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin heavy chain; Ferritin H subunit; Cell proliferation-inducing gene 15 protein

  • Research Field

    Neuroscience

[1]. Treffry A, et al. Dinuclear center of ferritin: studies of iron binding and oxidation show differences in the two iron sites. Biochemistry. 1997 Jan 14;36(2):432-41. [Content Brief]

[2]. Mancias JD, et al. Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy. Nature. 2014 May 1;509(7498):105-9. [Content Brief]

[3]. Mancias JD, et al. Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis. Elife. 2015 Oct 5;4:. [Content Brief]

Ferritin Heavy Chain Antibody (YA443) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Ferritin Heavy Chain (HY-P80670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ferritin Heavy Chain Antibody (YA443) Product Image
    1/16
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Ferritin Heavy Chain Antibody (YA443) Product Image
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Ferritin Heavy Chain (HY-P80670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Experimental Validation Results for Ferritin Heavy Chain Antibody (YA443)
  • Ferritin Heavy Chain Antibody (YA443) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Ferritin Heavy Chain (HY-P80670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ferritin Heavy Chain Antibody (YA443) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ferritin Heavy Chain Antibody (YA443) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Ferritin Heavy Chain with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ferritin Heavy Chain Antibody (HY-P80670) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ferritin Heavy Chain Antibody (YA443) Product Image WB
  • Ferritin Heavy Chain Antibody (YA443) Product Image ICC
  • Ferritin Heavy Chain Antibody (YA443) Product Image ICC