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Biglycan Antibody (YA2307)

(Synonyms: BGN; Biglycan; DSPG1; PG S1; PGI; SEMDX; SLRR1A)
Customer Review

Based on 1 Customer Validation

Biglycan Antibody (YA2307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Biglycan.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Biglycan Antibody (YA2307) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

Biglycan Antibody (YA2307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Biglycan.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Biglycan aa320-368.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Biglycan Antibody (YA2307)
    Western blot analysis was performed on extracts from C2C12 (lane 1, 15 μg) using Biglycan Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Biglycan Antibody (YA2307)
    Western blot analysis of extracts from K562(lane 2(20μg)), THP-1(lane 3(20μg)), C6(lane 4(20μg)) and RAW264.7(lane 5(20μg)) using Biglycan Antibody（HY-P82562）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Biglycan may be involved in collagen fiber assembly

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:Detected in placenta (at protein level) (PubMed:32337544) . Found in several connective tissues, especially in articular cartilages

  • Subunit

    Homodimer.

  • SwissProt ID

    P21810

  • Gene ID
    633 [NCBI]
  • Synonyms

    BGN; Biglycan; DSPG1; PG S1; PGI; SEMDX; SLRR1A

  • Research Field

    Cell Biology

Biglycan Antibody (YA2307) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from C2C12 (lane 1, 15 μg) using Biglycan Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg)), THP-1(lane 3(20μg)), C6(lane 4(20μg)) and RAW264.7(lane 5(20μg)) using Biglycan Antibody（HY-P82562）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Biglycan Antibody (YA2307) Product Image
    1/16
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Biglycan Antibody (YA2307) Product Image
  • Experimental Validation Results for Biglycan Antibody (YA2307)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from C2C12 (lane 1, 15 μg) using Biglycan Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Biglycan Antibody (YA2307)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg)), THP-1(lane 3(20μg)), C6(lane 4(20μg)) and RAW264.7(lane 5(20μg)) using Biglycan Antibody（HY-P82562）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Experimental Validation Results for Biglycan Antibody (YA2307)
  • Biglycan Antibody (YA2307) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from C2C12 (lane 1, 15 μg) using Biglycan Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Biglycan Antibody (YA2307) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg)), THP-1(lane 3(20μg)), C6(lane 4(20μg)) and RAW264.7(lane 5(20μg)) using Biglycan Antibody（HY-P82562）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Biglycan Antibody (YA2307) Product Image WB
  • Biglycan Antibody (YA2307) Product Image WB