Biglycan Antibody (YA2307)

Cat. No.: HY-P82562: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Biglycan Antibody (YA2307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Biglycan.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Biglycan Antibody (YA2307) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Biglycan Antibody (YA2307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Biglycan.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P21810

Gene ID

633 [NCBI]

Immunogen

A synthesized peptide derived from human Biglycan aa320-368.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from C2C12 (lane 1, 15 μg) using Biglycan Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from K562(lane 2(20μg)), THP-1(lane 3(20μg)), C6(lane 4(20μg)) and RAW264.7(lane 5(20μg)) using Biglycan Antibody（HY-P82562）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

Function：May be involved in collagen fiber assembly

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:Detected in placenta (at protein level) (PubMed:32337544) . Found in several connective tissues, especially in articular cartilages

Subunit：Homodimer.

Database

Entrez Gene: 633 Human ; 12111 Mouse ; 25181 Rat

SwissProt: P21810 Human ; P28653 Mouse ; P47853 Rat

OMIM: 300989 Human

Research Field

Cell Biology

Synonyms

BGN; Biglycan; DSPG1; PG S1; PGI; SEMDX; SLRR1A

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Biglycan Antibody (YA2307) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Biglycan Antibody (YA2307)

Biglycan Antibody (YA2307) Featured Recommendations:

Product Detail

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Background

Documentation

Biglycan Antibody (YA2307) Related Classifications

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