Actin Muscle Specific Antibody (YA5520)
(Synonyms: Actin, alpha cardiac muscle 1; Alpha-cardiac actin; Actin, alpha skeletal muscle; Alpha-actin-1; Actin, gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin)Actin Muscle Specific Antibody (YA5520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Actin Muscle Specific.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat, Monkey, Bovin, Pig, Chick
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Actin Muscle Specific Antibody (YA5520) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Actin Muscle Specific.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Monkey, Bovin, Pig, Chick
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Synthesized peptide derived from human Actin, Muscle Specific AA range: 2-50
Protein G
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Actin Muscle Specific is a P68032: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells
P68133: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells
P63267: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells
P62736: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells -
Subcellular Localization
P68032: Cytoplasm, cytoskeleton
P68133: Cytoplasm, cytoskeleton
P63267: Cytoplasm, cytoskeleton
P62736: Cytoplasm, cytoskeleton -
Expression
Tissue_specificity: P63267: In the intestine, abundantly expressed in smooth muscle cells of muscularis mucosa and muscularis propria. Also detected in intestinal vascular smooth muscle cells
Induction: P62736: Up-regulated in response to enterovirus 71 (EV71) infection -
Isoforms & Post-Translational Modification
P63267 has 2 isomers: P63267-1: 41877 Da (predicted); P63267-2: 37083 Da (predicted).
Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization;N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP;Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration;Methylated at His-74 by SETD3;(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148) -
Subunit
P68032: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others
P68133: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
P63267: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others
P62736: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others -
Synonyms
Actin, alpha cardiac muscle 1; Alpha-cardiac actin; Actin, alpha skeletal muscle; Alpha-actin-1; Actin, gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
Documentation
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