Lyn Antibody (YA7564)
(Synonyms: JTK8, LYN, Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn)Lyn Antibody (YA7564) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Lyn.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Lyn Antibody (YA7564) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Lyn.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Lyn is a Src-family tyrosine kinase that relays immune-cell receptor inputs to regulate proliferation, differentiation, apoptosis, migration, and metabolism[1]. Mechanistically, Lyn phosphorylates ITAM and ITIM signaling motifs, enabling both activating and inhibitory immunoreceptor signaling[1][2]. In B-cell biology, Lyn negatively regulates B-cell receptor signaling, and loss of Lyn in B cells leads to lupus-like autoimmunity in mouse models[3]. Compared with related Lyn isoforms, LynB shows a dominant immunosuppressive function in vivo, whereas LynA is uniquely required to restrain autoimmunity in female mice[4]. For experimental applications, bafetinib/NS-187 is a dual Bcr-Abl/Lyn tyrosine kinase inhibitor used in imatinib-resistant leukemia research[5]. MLR-1023 is an allosteric Lyn kinase activator and insulin receptor potentiator used in glucose-homeostasis models[6].
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Subcellular Localization
Cell membrane,Nucleus,Cytoplasm,Cytoplasm, perinuclear region,Golgi apparatus,Membrane
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Expression
Tissue_Specificity: Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors -
Isoforms & Post-Translational Modification
P07948 has two isomers: P07948-1: 58574 Da (predicted); P07948-2: 56033 Da (predicted).
Ubiquitinated by CBL, leading to its degradation丨Autophosphorylated (PubMed:18056483, PubMed:18070987, PubMed:7935444, PubMed:9171348, PubMed:9341198) -
Subunit
Interacts with TEC
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SwissProt ID
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Synonyms
JTK8, LYN, Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn
Documentation
References
[1]. Ingley E. Functions of the Lyn tyrosine kinase in health and disease. Cell Commun Signal. 2012 Jul 17;10(1):21. doi: 10.1186/1478-811X-10-21. PMID: 22805580; PMCID: PMC3464935. et al. Functions of the Lyn tyrosine kinase in health and disease. Cell Commun Signal. 2012 Jul 17;10(1):21. [Content Brief]
[2]. Xu Y, et al. Lyn tyrosine kinase: accentuating the positive and the negative. Immunity. 2005 Jan;22(1):9-18. [Content Brief]
[3]. Lamagna C, et al. B cell-specific loss of Lyn kinase leads to autoimmunity. J Immunol. 2014 Feb 1;192(3):919-28. [Content Brief]
[4]. Brian BF 4th, et al. A dominant function of LynB kinase in preventing autoimmunity. Sci Adv. 2022 Apr 22;8(16):eabj5227. [Content Brief]
[5]. Kimura S, et al. NS-187, a potent and selective dual Bcr-Abl/Lyn tyrosine kinase inhibitor, is a novel agent for imatinib-resistant leukemia. Blood. 2005 Dec 1;106(12):3948-54. [Content Brief]
[6]. Ochman AR, et al. The Lyn kinase activator MLR-1023 is a novel insulin receptor potentiator that elicits a rapid-onset and durable improvement in glucose homeostasis in animal models of type 2 diabetes. J Pharmacol Exp Ther. 2012 Jul;342(1):23-32. [Content Brief]
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