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  4. Monoclonal Antibodies Recombinant Antibodies
  5. TIMELESS Antibody (YA2204)

TIMELESS Antibody (YA2204)

Cat. No.: HY-P82459
COA
SDS
User Guide for Antibodies Technical Support

TIMELESS Antibody (YA2204) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIMELESS.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

TIMELESS Antibody (YA2204) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIMELESS.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 139 kDa;
Observed band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Timeless
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:20-1:50
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed:17141802, PubMed:17296725, PubMed:23359676, PubMed:23418588, PubMed:26344098, PubMed:31138685, PubMed:32705708, PubMed:35585232, PubMed:9856465). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN: this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed:17141802, PubMed:17296725, PubMed:23359676, PubMed:35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed:23359676). TIMELESS promotes TIPIN nuclear localization (PubMed:17141802, PubMed:17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G-quadruplex (G4) structures, possibly together with DDX1 (PubMed:32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed:17141802, PubMed:17296725, PubMed:26344098, PubMed:30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed:26344098, PubMed:30356214, PubMed:31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed:15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed:23418588, PubMed:31138685). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed:31138685, PubMed:9856465). May play a role as destabilizer of the PER2-CRY2 complex (PubMed:31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity)
Subcellular Localization：Nucleus; Chromosome
Expression：
Tissue_specificity:Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis

Induction:Regulated by the cell cycle. High levels in S, G (2) and M phases, with highest level in S phase. Low expression in G (0) and G (1) phases
Isoforms & Post-Translational Modification：Q9UNS1 has 2 isomers: Q9UNS1-1: 138658 Da (predicted); Q9UNS1-2: 138530 Da (predicted).
Subunit：Monomer (PubMed:28334766). Homodimer or homomultimer (By similarity). Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSKN1D and/or CSNK1E, TIMELESS, and the PER proteins (PubMed:9856465). Interacts with PER2 (PubMed:31138685). The interaction with PER2 is direct and via its second PAS domain (By similarity). Interacts directly with PER1 and PER3 (By similarity). Interacts with CRY2, CHEK1, ATR and ATRIP (PubMed:15798197, PubMed:31138685). Interacts with CRY1 (By similarity) (PubMed:15798197). Interacts with CLSPN; the interaction is required for leading-strand replication (PubMed:17141802, PubMed:23359676, PubMed:34694004, PubMed:34700328, PubMed:35585232). Interacts (via N-terminus) with TIPIN (PubMed:17102137, PubMed:17116885, PubMed:17296725, PubMed:28334766, PubMed:34694004, PubMed:34700328). The TIMELESS-TIPIN heterodimer binds preferably to guanine-rich quadruplex-forming (G4) DNA structures (PubMed:32705708). Associates with the MCM2-7 complex (PubMed:23359676, PubMed:34694004, PubMed:34700328). Interacts with DNA polymerases alpha, delta and epsilon (PubMed:23359676). Interacts with DDX11; this interaction increases recruitment of both proteins onto chromatin in response to replication stress induction by hydroxyurea (PubMed:20124417, PubMed:26503245, PubMed:32705708). Interacts with PARP1; interaction is direct and independent of poly-ADP-ribose (PubMed:26344098, PubMed:30356214)
Database

Entrez Gene: 8914 Human

SwissProt: Q9UNS1 Human

OMIM: 620015 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
hTIM; TIM; TIM1; Timeless; timeless circadian clock 1; TIMELESS1
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TIMELESS Antibody (YA2204) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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