Copy Link Print Information

Pyrophosphatase 1 Antibody (YA1786)

(Synonyms: PP; PP1; IOPPP; HEL-S-66p; SID6-8061)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Pyrophosphatase 1 Antibody (YA1786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyrophosphatase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Pyrophosphatase 1 Antibody (YA1786)
  • Pyrophosphatase 1 Antibody (YA1786) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Pyrophosphatase 1 Antibody (YA1786)

More

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:20 1:10-1:100

Product Details

Description

Pyrophosphatase 1 Antibody (YA1786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyrophosphatase 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity Database
Immunogen

A synthetic peptide of human Pyrophosphatase 1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pyrophosphatase 1 Antibody (YA1786)
    Western blot analysis was performed on protein extracts (25 μg) from Rat lung (lane 1), Rat kidney (lane 2), Rat liver (lane 3), and 293T (lane 4) using Pyrophosphatase 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Pyrophosphatase 1 catalyzes the hydrolysis of inorganic diphosphate into two phosphate molecules. Has MAP kinase serine/threonine phosphatase activity toward c-Jun N-terminal kinase (JNK) and, in neuronal cells, is involved in the negative regulation of neurite growth via JNK dephosphorylation[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expressed ubiquitously

  • Subunit

    Monomer. Homodimer

  • SwissProt ID

    Q15181

  • Gene ID
    5464 [NCBI]
  • Synonyms

    PP; PP1; IOPPP; HEL-S-66p; SID6-8061

  • Research Field

    Signal Transduction

References

[1]. Hu F, et al. Structural and biochemical characterization of inorganic pyrophosphatase from Homo sapiens. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1115-1121. [Content Brief]

[2]. Zheng S, et al. Structural and biochemical characterization of active sites mutant in human inorganic pyrophosphatase. Biochim Biophys Acta Gen Subj. 2024 May;1868(5):130594. [Content Brief]

Pyrophosphatase 1 Antibody (YA1786) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat lung (lane 1), Rat kidney (lane 2), Rat liver (lane 3), and 293T (lane 4) using Pyrophosphatase 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Pyrophosphatase 1 Antibody (YA1786) Product Image
    1/16
  • Experimental Validation Results for Pyrophosphatase 1 Antibody (YA1786)
  • Pyrophosphatase 1 Antibody (YA1786) Product Image
  • Experimental Validation Results for Pyrophosphatase 1 Antibody (YA1786)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat lung (lane 1), Rat kidney (lane 2), Rat liver (lane 3), and 293T (lane 4) using Pyrophosphatase 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Pyrophosphatase 1 Antibody (YA1786)
  • Pyrophosphatase 1 Antibody (YA1786) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Rat lung (lane 1), Rat kidney (lane 2), Rat liver (lane 3), and 293T (lane 4) using Pyrophosphatase 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Pyrophosphatase 1 Antibody (YA1786) Product Image WB