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Collagen I alpha 2 Antibody (YA3695)

(Synonyms: OI4; EDSCV; EDSARTH2)
Customer Review

Based on 1 Customer Validation

Collagen I alpha 2 Antibody (YA3695) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Collagen I alpha 2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Collagen I alpha 2 Antibody (YA3695) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Collagen I alpha 2 Antibody (YA3695) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Collagen I alpha 2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 129 kDa
Immunogen

Purified recombinant fragment of human COL1A2 (AA: 23-79) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718809

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    Flow cytometric analysis of 1X106 HeLa cells labeling Collagen I alpha 2 Antibody (HY-P83998, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    Immunocytochemistry analysis of HeLa cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    Immunocytochemistry analysis of NIH-3T3 cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Collagen I alpha 2 is a Type I collagen is a member of group I collagen (fibrillar forming collagen)

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:The fibers that make up tendons, ligaments, and bones. In bones, these fibers are mineralized by hydroxyapatite calcium.

  • Subunit

    Trimers of one alpha 2(I) and two alpha 1(I) chains (Probable). Interacts (via C-terminus) with TMEM131 (via PapD-L domain); the interaction is direct and is involved in assembly and TRAPPIII ER-to-Golgi transport complex-dependent secretion of collagen (PubMed:32095531)

  • SwissProt ID

    P08123

  • Gene ID
    1278 [NCBI]
  • Synonyms

    OI4; EDSCV; EDSARTH2

Collagen I alpha 2 Antibody (YA3695) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Collagen I alpha 2 Antibody (HY-P83998, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen I alpha 2 Antibody (YA3695) Product Image
    1/16
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Collagen I alpha 2 Antibody (YA3695) Product Image
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Collagen I alpha 2 Antibody (HY-P83998, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Experimental Validation Results for Collagen I alpha 2 Antibody (YA3695)
  • Collagen I alpha 2 Antibody (YA3695) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling Collagen I alpha 2 Antibody (HY-P83998, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Collagen I alpha 2 Antibody (YA3695) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen I alpha 2 Antibody (YA3695) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling Collagen I alpha 2 with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Collagen I alpha 2 Antibody (HY-P83998) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Collagen I alpha 2 Antibody (YA3695) Product Image FC
  • Collagen I alpha 2 Antibody (YA3695) Product Image ICC
  • Collagen I alpha 2 Antibody (YA3695) Product Image ICC