PAR4 Antibody (YA5006)(PBS only)

Cat. No.: HY-P85314A: Data Sheet User Guide for Antibodies
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PAR4 Antibody (YA5006) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PAR4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PAR4 Antibody (YA5006) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PAR4.

Host

Mouse

Molecular Weight

Predicted band size: 37 kDa;

Species Reactivity

Human

SwissProt ID

Q96IZ0

Gene ID

5074 [NCBI]

Immunogen

Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Also seems to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Widely expressed. Elevated expression is found in a variety of neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) , Alzheimer's disease, Parkinson's disease, Huntington's disease, and stroke. Downregulated expression is found in a variety of cancers.

Induction:By apoptosis

Subunit：Homooligomer. Interacts (via the C-terminal region) with WT1 (PubMed:8943350). Interacts with THAP1 (PubMed:12717420). Interacts with AATF (PubMed:14627703). Interacts with BACE1 (PubMed:15671026).

Synonyms

PAWR; PAR4; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Par-4

Documentation

User Guide for Antibodies (1077 KB)