HKDC1 Antibody (YA2988)

Cat. No.: HY-P83243: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

HKDC1 Antibody (YA2988) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HKDC1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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HKDC1 Antibody (YA2988) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

HKDC1 Antibody (YA2988) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HKDC1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 103 kDa;

Observed band size: 103 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q2TB90

Gene ID

80201 [NCBI]

Immunogen

Recombinant protein of human HKDC1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Ramos (lane 2, 15 μg), U-87MG (lane 3, 15 μg), 293T (lane 4, 15 μg), and A549 (lane 5, 15 μg) using HKDC1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (PubMed:30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed:30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity)

Subcellular Localization：Cytoplasm; Mitochondrion membrane; Peripheral membrane protein; Photoreceptor inner segment

Expression：
Tissue_specificity:Widely expressed (PubMed:27459389, PubMed:29401404) . Highly expressed in the brush border, surface epithelium and the myenteric plexus of the small and large intestines; the acinar centrocytes and interlobular ducts of the pancreas; and the alveolar macrophages in the lungs (at protein level) (PubMed:29401404) . Present at moderate level in the thyroid follicular epithelium (at protein level) (PubMed:29401404)

Isoforms & Post-Translational Modification：Q2TB90 has 3 isomers: Q2TB90-1: 102545 Da (predicted); Q2TB90-2: 90575 Da (predicted); Q2TB90-3: 82682 Da (predicted).

Database

Entrez Gene: 80201 Human

SwissProt: Q2TB90 Human

OMIM: 619614 Human

Research Field

Signal Transduction

Synonyms

Hexokinase domain-containing protein 1

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

HKDC1 Antibody (YA2988) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HKDC1 Antibody (YA2988)

HKDC1 Antibody (YA2988) Featured Recommendations:

Product Detail

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Background

Documentation

HKDC1 Antibody (YA2988) Related Classifications

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