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AK3L1 Antibody (YA2693)

(Synonyms: AK3; AK 4; AK3L1; AK3L2)
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Based on 1 Customer Validation

AK3L1 Antibody (YA2693) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AK3L1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for AK3L1 Antibody (YA2693)
  • AK3L1 Antibody (YA2693) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:5000 1:50-1:100 1:200-1:500

Product Details

Description

AK3L1 Antibody (YA2693) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AK3L1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 205 Human

SwissProt: P27144 Human

Immunogen

A synthetic peptide of human AK3L1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AK3L1 Antibody (YA2693)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), U87 (lane 3), A549 (lane 4), Raji (lane 5), HT-1080 (lane 6), THP-1 (lane 7), and MCF-7 (lane 8) using AK3L1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    AK3L1 is a Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions. Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP. Phosphorylates only AMP when using GTP as phosphate donor. In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor. Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress[1][2][3][4][5][6][7].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_specificity:Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain

    Induction:By hypoxia (at protein level)

  • Subunit

    Monomer (Ref.17). Interacts with SLC25A5/ANT2 (PubMed:19130895)

  • SwissProt ID

    P27144

  • Gene ID
    205 [NCBI]
  • Synonyms

    AK3; AK 4; AK3L1; AK3L2

  • Research Field

    Signal Transduction

AK3L1 Antibody (YA2693) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), U87 (lane 3), A549 (lane 4), Raji (lane 5), HT-1080 (lane 6), THP-1 (lane 7), and MCF-7 (lane 8) using AK3L1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • AK3L1 Antibody (YA2693) Product Image
    1/16
  • Experimental Validation Results for AK3L1 Antibody (YA2693)
  • AK3L1 Antibody (YA2693) Product Image
  • Experimental Validation Results for AK3L1 Antibody (YA2693)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), U87 (lane 3), A549 (lane 4), Raji (lane 5), HT-1080 (lane 6), THP-1 (lane 7), and MCF-7 (lane 8) using AK3L1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for AK3L1 Antibody (YA2693)
  • AK3L1 Antibody (YA2693) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), U87 (lane 3), A549 (lane 4), Raji (lane 5), HT-1080 (lane 6), THP-1 (lane 7), and MCF-7 (lane 8) using AK3L1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • AK3L1 Antibody (YA2693) Product Image WB