FUT8 Antibody
(Synonyms: FUT8 Antibody)FUT8 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to FUT8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:50-1:100 |
Product Details
FUT8 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to FUT8.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
Synthetic peptide of human FUT8.
Endogenous
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FUT8 catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. Fucosylates the reducing GlcNAc residue in complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs. Fully converts Fc glycoforms containing one or two terminal GlcNAc moieties (G0-GlcNAc and G0)[1][2][3][4].
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Subcellular Localization
Golgi apparatus, Golgi stack membrane
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Isoforms & Post-Translational Modification
FUT8 has 4 isoforms, Q9BYC5-1: amino acid length is 575, molecular weight is 66516 Da (predicted); Q9BYC5-2: amino acid length is 308, molecular weight is 35795 Da (predicted); Q9BYC5-3: amino acid length is 412, molecular weight is 47255 Da (predicted); Q9BYC5-4: amino acid length is 169, molecular weight is 19520 Da (predicted).Tyrosine phosphorylated by PKDCC/VLK
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SwissProt ID
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Synonyms
FUT8 Antibody
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Research Field
Cell Biology
Documentation
[1]. Ihara H, et al. Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8. Glycobiology. 2007 May;17(5):455-66. [Content Brief]
[2]. Ng BG, et al. Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation. Am J Hum Genet. 2018 Jan 4;102(1):188-195. [Content Brief]
[3]. Jaroentomeechai T, et al. A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nat Commun. 2022 Oct 24;13(1):6325. [Content Brief]
[4]. Yanagidani S, et al. Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells. J Biochem. 1997 Mar;121(3):626-32. [Content Brief]
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