KLHL2 Antibody (YA8500)(PBS only)

Cat. No.: HY-P88816A: Data Sheet User Guide for Antibodies
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KLHL2 Antibody (YA8500) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLHL2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KLHL2 Antibody (YA8500) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLHL2.

Host

Mouse

Molecular Weight

Predicted band size: 65.8 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O95198

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, WNK1, WNK3 and WNK4, leading most often to their proteasomal degradation (PubMed:23838290). The BCR(KLHL2) complex catalyzes ubiquitination and degradation of NPTXR (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4 (PubMed:23838290). Plays a role in the reorganization of the actin cytoskeleton (PubMed:10397770). Promotes growth of cell projections in oligodendrocyte precursors (PubMed:15715669)

Subcellular Localization：Cytoplasm, cytoskeleton,Cell projection, ruffle,Cell projection,Cell projection, lamellipodium,Cytoplasm, cytosol

Expression：
Tissue_Specificity: Ubiquitous. Detected throughout the brain

Isoforms & Post-Translational Modification：O95198 has three isomers: O95198-1: 65975 Da (predicted); O95198-2: 66624 Da (predicted); O95198-3: 56086 Da (predicted).

Subunit：Component of the BCR(KLHL2) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL2 and RBX1 (PubMed:23838290)

Synonyms

ABP-KELCH; MAV; MAYVEN

Documentation

User Guide for Antibodies (1077 KB)