Emerin Antibody (YA6564)

Cat. No.: HY-P86871: Data Sheet User Guide for Antibodies
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Emerin Antibody (YA6564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Emerin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Emerin Antibody (YA6564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Emerin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 29 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P50402

Gene ID

2010 [NCBI]

Immunogen

Recombinant protein within human Emerin aa 1-200 / 254.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells (PubMed:32923640). EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD

Subcellular Localization：Nucleus inner membrane,Nucleus outer membrane

Expression：
Tissue_Specificity: Skeletal muscle, heart, colon, testis, ovary and pancreas

Isoforms & Post-Translational Modification：P50402: 254 amino acids, molecular weight 28994 Da.
Acetylation is generally linked to gene activation丨Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription丨Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation丨Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation丨Phosphorylated at Thr-4 (H3T3ph) by VRK1 (PubMed:31527692)丨Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity)丨Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2丨Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells丨Butyrylation of histones marks active promoters and competes with histone acetylation丨Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711)丨Serine ADP-ribosylation by PARP1 or PARP2 constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210, PubMed:34874266)丨Serotonylated by TGM2 at Gln-6 (H3Q5ser) during serotonergic neuron differentiation (PubMed:30867594)丨Dopaminylated by TGM2 at Gln-6 (H3Q5dop) in ventral tegmental area (VTA) neurons (PubMed:32273471)丨Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription

Subunit：Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521

Synonyms

EDMD antibody; Emd antibody; EMD_HUMAN antibody; Emerin antibody; Emery Dreifuss muscular dystrophy antibody; STA antibody;

Documentation

User Guide for Antibodies (1077 KB)