LARP1 Antibody (YA6634)

Cat. No.: HY-P86941: Data Sheet User Guide for Antibodies
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LARP1 Antibody (YA6634) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LARP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

LARP1 Antibody (YA6634) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LARP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 124 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

Q6PKG0

Gene ID

23367 [NCBI]

Immunogen

Recombinant protein within human LARP1 aa 181-330.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:26206669, PubMed:28379136, PubMed:28650797, PubMed:29244122). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28379136, PubMed:28650797, PubMed:29244122). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543)|(Microbial infection) Positively regulates the replication of dengue virus (DENV)

Subcellular Localization：Cytoplasm,Cytoplasmic granule

Expression：Induction: Up-regulated in a number of hepatocellular carcinoma cell lines and liver cancer lesions, as well as in patients with hepatocellular carcinoma with a lower survival rate (at protein level)

Isoforms & Post-Translational Modification：Q6PKG0 has two isomers: Q6PKG0-1: 123510 Da (predicted); Q6PKG0-3: 116465 Da (predicted).
Phosphorylated on multiple Ser and Thr residues in response to active mTORC1

Subunit：Interacts with PABPC1/PABP (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797)

Synonyms

1810024J12Rik antibody; 3110040D16Rik antibody; KIAA0731 antibody; La related protein antibody; La ribonucleoprotein domain family, member 1 antibody; LARP antibody; MGC19556 antibody; mKIAA0731 antibody; rCG_32791 antibody; RGD1306683 antibody; 1810024J12Rik antibody; 3110040D16Rik antibody; KIAA0731 antibody; La related protein antibody; La ribonucleoprotein domain family, member 1 antibody; LARP antibody; MGC19556 antibody; mKIAA0731 antibody; rCG_32791 antibody; RGD1306683 antibody; RP23-293I3.7 antibody;

Documentation

User Guide for Antibodies (1077 KB)