MICB Antibody
(Synonyms: PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B)MICB Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MICB.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:1000-2000 |
Product Details
MICB Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MICB.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 4; 37; 42 kDa
Purified recombinant protein of human MICB.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MICB is widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production[1][2].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue.
Induction: By heat shock, oxidative stress, retinoic acid, IFN-alpha and the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine. Induction by IFN-alpha is impaired in patients with chronic hepatitis C virus infection. Down-regulated by human cytomegalovirus UL112 microRNA during viral infection which leads to decreased binding of KLRK1/NKG2D and reduced killing by natural killer cells. -
Isoforms & Post-Translational Modification
MICB has 3 isoforms, Q29980-1: amino acid length is 383, molecular weight is 42575 Da (predicted); Q29980-2: amino acid length is 340, molecular weight is 37580 Da (predicted); Q29980-3: amino acid length is 41, molecular weight is 4287 Da (predicted).
Proteolytically cleaved and released from the cell surface of tumor cells. -
Subunit
Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin.
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SwissProt ID
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Synonyms
PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B
Documentation
[1]. Steinle A, et al. Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family. Immunogenetics. 2001 May-Jun;53(4):279-87. [Content Brief]
[2]. Sutherland CL, et al. UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells. J Immunol. 2002 Jan 15;168(2):671-9. [Content Brief]
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