BRAT1 Antibody (YA2294)
(Synonyms: BRCA1-associated ATM activator 1; BRCA1-associated protein required for ATM activation protein 1)BRAT1 Antibody (YA2294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
BRAT1 Antibody (YA2294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 88 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 88 kDa
Recombinant protein of human BRAT1 aa712-819.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BRAT1 component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex. Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex. Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer. In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. Plays a role in regulating mitochondrial function and cell proliferation. Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:general expression -
Isoforms & Post-Translational Modification
Q6PJG6 has 3 isomers: Q6PJG6-1: 88119 Da (predicted); Q6PJG6-2: 30560 Da (predicted); Q6PJG6-3: 31676 Da (predicted).
Ubiquitinated by NEDD4, NEDD4L and ITCH; mono- and polyubiquitinated forms are detected -
Subunit
Part of the multiprotein complex composed of BRAT1, WDR73, as well as integrator complex subunits INTS9 and INTS11 (PubMed:39032489). Interacts with BRCA1 and ATM (PubMed:16452482, PubMed:22977523). Interacts with MTOR and RPTOR (PubMed:25657994). Interacts with NDFIP1 (PubMed:25631046). Interacts with SMC1A and PRKDC (PubMed:22977523)
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SwissProt ID
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Synonyms
BRCA1-associated ATM activator 1; BRCA1-associated protein required for ATM activation protein 1
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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