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  5. BRAT1 Antibody (YA2294)

BRAT1 Antibody (YA2294)

Cat. No.: HY-P82549
User Guide for Antibodies Technical Support

BRAT1 Antibody (YA2294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.

For research use only. We do not sell to patients.

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20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

BRAT1 Antibody (YA2294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 88 kDa;
Observed band size: 88 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human BRAT1 aa712-819.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex (PubMed:39032489, PubMed:39032490). Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex (PubMed:39032489, PubMed:39032490). Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer (PubMed:39032489). In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6) (PubMed:39032489). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed:16452482, PubMed:22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed:25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994)
Subcellular Localization：Nucleus; Cytoplasm
Expression：
Tissue_specificity:general expression
Isoforms & Post-Translational Modification：Q6PJG6 has 3 isomers: Q6PJG6-1: 88119 Da (predicted); Q6PJG6-2: 30560 Da (predicted); Q6PJG6-3: 31676 Da (predicted).
Ubiquitinated by NEDD4, NEDD4L and ITCH; mono- and polyubiquitinated forms are detected
Subunit：Part of the multiprotein complex composed of BRAT1, WDR73, as well as integrator complex subunits INTS9 and INTS11 (PubMed:39032489). Interacts with BRCA1 and ATM (PubMed:16452482, PubMed:22977523). Interacts with MTOR and RPTOR (PubMed:25657994). Interacts with NDFIP1 (PubMed:25631046). Interacts with SMC1A and PRKDC (PubMed:22977523)
Database

Entrez Gene: 221927 Human

SwissProt: Q6PJG6 Human

OMIM: 614498 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
BRCA1-associated ATM activator 1; BRCA1-associated protein required for ATM activation protein 1
Documentation
User Guide for Antibodies (1077 KB)

BRAT1 Antibody (YA2294) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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