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ANPR B Antibody (YA2913)

(Synonyms: AMDM; ANPB; ANPRB; GCB; GUC2B; GUCY2B; Npr2; NPRB; NPRBi)
Customer Review

Based on 1 Customer Validation

ANPR B Antibody (YA2913) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ANPR B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ANPR B Antibody (YA2913)
  • ANPR B Antibody (YA2913) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ANPR B Antibody (YA2913) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ANPR B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 117 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 117 kDa
Species Reactivity Database

Entrez Gene: 4882 Human

SwissProt: P20594 Human

Immunogen

A synthesized peptide derived from human ANPRB aa801-856.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105283

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ANPR B Antibody (YA2913)
    Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), HuH-7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using NPR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ANPR B receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    P20594 has 2 isomers: P20594-1: 117022 Da (predicted); P20594-2: 111208 Da (predicted).
    Phosphorylated (PubMed:26980729). Phosphorylation of the protein kinase-like domain is required for full activation by CNP (By similarity);Glycosylated

  • SwissProt ID

    P20594

  • Gene ID
    4882 [NCBI]
  • Synonyms

    AMDM; ANPB; ANPRB; GCB; GUC2B; GUCY2B; Npr2; NPRB; NPRBi

  • Research Field

    Neuroscience

ANPR B Antibody (YA2913) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), HuH-7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using NPR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ANPR B Antibody (YA2913) Product Image
    1/16
  • Experimental Validation Results for ANPR B Antibody (YA2913)
  • ANPR B Antibody (YA2913) Product Image
  • Experimental Validation Results for ANPR B Antibody (YA2913)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), HuH-7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using NPR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ANPR B Antibody (YA2913)
  • ANPR B Antibody (YA2913) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), HuH-7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using NPR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ANPR B Antibody (YA2913) Product Image WB