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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. ANPR B Antibody (YA2913)

ANPR B Antibody (YA2913) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ANPR B.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

Descripciòn

ANPR B Antibody (YA2913) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ANPR B.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 117 kDa;
Observed band size: 117 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human ANPRB aa801-856.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), HuH-7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using NPR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth
Subcellular Localization：Cell membrane; Single-pass type I membrane protein
Isoforms & Post-Translational Modification：P20594 has 2 isomers: P20594-1: 117022 Da (predicted); P20594-2: 111208 Da (predicted).
Phosphorylated (PubMed:26980729). Phosphorylation of the protein kinase-like domain is required for full activation by CNP (By similarity);Glycosylated
RRID
Database

Entrez Gene: 4882 Human

SwissProt: P20594 Human

OMIM: 108961 Human

Research Field

Neuroscience
Synonyms
AMDM; ANPB; ANPRB; GCB; GUC2B; GUCY2B; Npr2; NPRB; NPRBi
Documentación
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ANPR B Antibody (YA2913) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
ANPR B Antibody (YA2913)
Cat. No.:
HY-P83168
Cantidad:
MCE Japan Authorized Agent:

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