Myosin Light Chain 2 Antibody (YA278)

Cat. No.: HY-P80235: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Myosin Light Chain 2 Antibody (YA278) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Light Chain 2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Myosin Light Chain 2 Antibody (YA278) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Myosin Light Chain 2 Antibody (YA278) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Light Chain 2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 19 kDa;

Observed band size: 19 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P10916

Gene ID

4633 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Myosin Light Chain 2.AA range:117-166.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from M-muscle (lane 1) and M-spleen (lane 2) and M-heart (lane 3) using Myosin Light Chain 2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity)

Subcellular Localization：Cytoplasm, myofibril, sarcomere, A band

Expression：
Tissue_specificity:High expression in type I myofibrils

Subunit：Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC (PubMed:11773029)

RRID

AB_3102099

Database

Entrez Gene: 4633 Human ; 17906 Mouse ; 363925 Rat

SwissProt: P10916 Human ; P51667 Mouse ; P08733 Rat

OMIM: 608758 Human

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Myosin Light Chain 2 Antibody (YA278) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Myosin Light Chain 2 Antibody (YA278)

Myosin Light Chain 2 Antibody (YA278) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

Myosin Light Chain 2 Antibody (YA278) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report