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  5. PU.1/SPI1 Antibody (YA117)

PU.1/SPI1 Antibody (YA117)

Cat. No.: HY-P80288
User Guide for Antibodies Technical Support

PU.1/SPI1 Antibody (YA117) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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Description

PU.1/SPI1 Antibody (YA117) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 31 kDa;
Observed band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human PU1/Spi1.AA range:1-50.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity)
Subcellular Localization：Nucleus
Expression：
Tissue_specificity:In the bone marrow, it is mainly concentrated in hematopoietic stem cells, lymphoid progenitor cells, myeloid cells (granulocyte-macrophage progenitor cells, classical dendritic cells, monocytes) , and B cell clusters. Among B cells, it is primarily expressed in pre-B1 cells (PubMed: 33951726) . It is also expressed in germinal center B cells (PubMed: 23166356) .

Induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155) (PubMed:23166356)
Isoforms & Post-Translational Modification：P17947 has 2 isomers: P17947-1: 31083 Da (predicted); P17947-2: 31211 Da (predicted).
Subunit：Binds DNA as a monomer. Can form homomers (PubMed:10196196). Directly interacts with CEBPD/NF-IL6-beta; this interaction does not affect DNA-binding properties of each partner (By similarity).
Database

Entrez Gene: 6688 Human

SwissProt: P17947 Human

OMIM: 619707 Human

Research Field

Immunology
Synonyms
PU.1,SPI1
Documentation
User Guide for Antibodies (1077 KB)

PU.1/SPI1 Antibody (YA117) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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