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PU.1/SPI1 Antibody (YA117)

(Synonyms: PU.1,SPI1)
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PU.1/SPI1 Antibody (YA117) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • PU.1/SPI1 Antibody (YA117) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

PU.1/SPI1 Antibody (YA117) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database

Entrez Gene: 6688 Human

SwissProt: P17947 Human

Immunogen

Synthetic peptide corresponding to Human PU1/Spi1.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    PU.1/SPI1 is a Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions. Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R. Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1. May be particularly important for the pro- to pre-B cell transition. Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box. In vitro can bind RNA and interfere with pre-mRNA splicing[1][2].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:In the bone marrow, it is mainly concentrated in hematopoietic stem cells, lymphoid progenitor cells, myeloid cells (granulocyte-macrophage progenitor cells, classical dendritic cells, monocytes) , and B cell clusters. Among B cells, it is primarily expressed in pre-B1 cells (PubMed: 33951726) . It is also expressed in germinal center B cells (PubMed: 23166356) .

    Induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155) (PubMed:23166356)

  • Isoforms & Post-Translational Modification

    P17947 has 2 isomers: P17947-1: 31083 Da (predicted); P17947-2: 31211 Da (predicted).

  • Subunit

    Binds DNA as a monomer. Can form homomers (PubMed:10196196). Directly interacts with CEBPD/NF-IL6-beta; this interaction does not affect DNA-binding properties of each partner (By similarity).

  • SwissProt ID

    P17947

  • Gene ID
    6688 [NCBI]
  • Synonyms

    PU.1,SPI1

  • Research Field

    Immunology

[1]. Pham TH, et al. Mechanisms of in vivo binding site selection of the hematopoietic master transcription factor PU.1. Nucleic Acids Res. 2013 Jul;41(13):6391-402. [Content Brief]

[2]. Le Coz C, et al. Constrained chromatin accessibility in PU.1-mutated agammaglobulinemia patients. J Exp Med. 2021 Jul 5;218(7):. [Content Brief]

PU.1/SPI1 Antibody (YA117) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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