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  5. beta II Tubulin Antibody (YA3010)

beta II Tubulin Antibody (YA3010)

Cat. No.: HY-P83265
User Guide for Antibodies Technical Support

beta II Tubulin Antibody (YA3010) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3010), targeting beta II Tubulin. It can be used as a loading control antibody.

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Tamaño Precio Stock Cantidad
20 μL Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Obtener un presupuesto  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Descripciòn

Descripciòn

beta II Tubulin Antibody (YA3010) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3010), targeting beta II Tubulin. It can be used as a loading control antibody.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Peso molecular
Predicted band size: 50 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human beta II Tubulin aa400-445/445.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Pureza Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Background
Function：P68371: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin
Q13885: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin
Q9BVA1: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:23001566, PubMed:26732629, PubMed:28013290). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. Plays a critical role in proper axon guidance in both central and peripheral axon tracts (PubMed:23001566). Implicated in neuronal migration (PubMed:19465910)
Subcellular Localization：P68371: Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, flagellum axoneme
Q13885: Cytoplasm, cytoskeleton
Q9BVA1: Cytoplasm, cytoskeleton
Expression：
Tissue_specificity: P68371: Ubiquitous
Q13885: High expression in brain, where it represents 30% of all beta-tubulins
Q9BVA1: High expression in brain
Isoforms & Post-Translational Modification：Human (P68371): 445 amino acids, molecular weight 49,831 Da.
Human (Q13885): 445 amino acids, molecular weight 49,907 Da.
Human (Q9BVA1): 445 amino acids, molecular weight 49,953 Da.
Some glutamate residues at the C-terminus are polyglutamylated. Phosphorylated on Ser-172 by CDK1 during the cell cycle.
Subunit：P68371: Dimer of alpha and beta chains.
Q13885: Interacts with ZNRF1 (By similarity).
Q9BVA1: Dimer of alpha and beta chains (PubMed:23001566, PubMed:26732629, PubMed:28013290).
Database

Entrez Gene: 10383 Human ; 347733 Human ; 7280 Human ; 22151 Mouse ; 227613 Mouse ; 73710 Mouse ; 291081 Rat ; 296554 Rat ; 498736 Rat

SwissProt: P68371 Human ; Q13885 Human ; Q9BVA1 Human ; P68372 Mouse ; Q7TMM9 Mouse ; Q9CWF2 Mouse ; P85108 Rat Q3KRE8

OMIM: 617879 Human

Research Field

Cell Biology
Synonyms
class II beta tubulin isotype; Beta2; Tubulin beta 2 chain; TUBB; TUBB2
Documentación
User Guide for Antibodies (1077 KB)

beta II Tubulin Antibody (YA3010) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
beta II Tubulin Antibody (YA3010)
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HY-P83265
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