Collagen II Antibody (YA5846)
(Synonyms: Collagen alpha-1; II; chain; Alpha-1 type II collagen; [Cleaved into: Collagen alpha-1; II; chain; Chondrocalcin])Collagen II Antibody (YA5846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen II.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, IP, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:100-1:500 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Collagen II Antibody (YA5846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen II.
-
Host Rabbit
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 200 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 142 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Collagen II is the major collagen synthesized by chondrocytes and a core structural component of articular cartilage, vitreous humour, inner ear, and nucleus pulposus[1]. Mechanistically, COL2A1 suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction and regulating BMP-SMAD1 signaling[2]. In disease models, COL2A1 loss accelerates chondrocyte hypertrophy, while degenerative human articular cartilage shows reduced COL2A1 with increased hypertrophic markers and BMP-SMAD1 activity[2]. Type II collagen degradation can also expose a cryptic peptide sequence that induces collagenase activity, MMP-13, and chondrocyte hypertrophy in articular cartilage explants[3]. Compared with type X collagen, which marks hypertrophic chondrocytes in osteoarthritis, collagen II better reflects the stable hyaline cartilage phenotype[4]. Compared with collagen I devices, collagen II biomaterials more directly match cartilage extracellular matrix composition and support cartilage engineering research[5]. For experimental applications, collagen II scaffolds help maintain or rejuvenate hyaline chondrocyte phenotype through Wnt-related mechanisms, supporting use in cartilage tissue engineering models[6].
-
Subcellular Localization
Secreted, extracellular space, extracellular matrix
-
Expression
Tissue_specificity:Isomer 2 is highly expressed in juvenile chondrocytes and lowly expressed in fetal chondrocytes. -
Isoforms & Post-Translational Modification
P02458 has 3 isomers: P02458-2: 141785 Da (predicted); P02458-1: 134389 Da (predicted); P02458-3: 29781 Da (predicted).
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2;Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains;Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline;Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains;O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide -
Subunit
Homotrimers of alpha 1(II) chains
-
SwissProt ID
-
Synonyms
Collagen alpha-1; II; chain; Alpha-1 type II collagen; [Cleaved into: Collagen alpha-1; II; chain; Chondrocalcin]
Documentation
References
[1]. Norhalifah HK, et al. The genetic history of Peninsular Malaysia. Gene. 2016 Jul 15;586(1):129-35. [Content Brief]
[2]. Lian C, et al. Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction. Bone Res. 2019 Mar 6;7:8. [Content Brief]
[3]. van der Kraan PM, et al. Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration? Osteoarthritis Cartilage. 2012 Mar;20(3):223-32. [Content Brief]
[4]. Wu Z, et al. Collagen type II: From biosynthesis to advanced biomaterials for cartilage engineering. Biomater Biosyst. 2021 Nov 22;4:100030. [Content Brief]
[5]. Zhou H, et al. Rejuvenating Hyaline Cartilaginous Phenotype of Dedifferentiated Chondrocytes in Collagen II Scaffolds: A Mechanism Study Using Chondrocyte Membrane Nanoaggregates as Antagonists. ACS Nano. 2024 Jan 23;18(3):2077-2090. [Content Brief]
Powered by Bioz
