Hsp40 Antibody

Hsp40 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 38 kDa, targeting to Hsp40. It can be used for WB assays with tag free, in the background of Human.

Hsp40: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Free

3337

Predicted band size: 38 kDa;Observed band size: 38 kDa

affinity purified

Non-conjugated

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

P25685

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using Hsp40(HY-P80710) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80710, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Hsp40.The exact sequence is proprietary to MCE.