ApoER2 Antibody (YA2128)(PBS only)
(Synonyms: LRP8; APOER2; Low-density lipoprotein receptor-related protein 8; LRP-8; Apolipoprotein E receptor 2)ApoER2 Antibody (YA2128) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ApoER2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Rat, Hamster
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
ApoER2 Antibody (YA2128) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ApoER2.
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Host Rabbit
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Species ReactivityHuman, Rat, Hamster
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Observed Molecular WeightObserved band size: 130,106 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 106 kDa
A synthetic peptide of human ApoER2
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), SK-BR-3 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using ApoER2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Background
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Function
ApoER2 cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2. Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells; (Microbial infection) Acts as a receptor for Semliki Forest virus[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:It is primarily expressed in the brain and placenta. It is also expressed in platelets and megakaryocytes. It is not expressed in the liver. -
Isoforms & Post-Translational Modification
Q14114 has 5 isomers: Q14114-1: 105634 Da (predicted); Q14114-2: 77845 Da (predicted); Q14114-3: 99092 Da (predicted); Q14114-4: 87959 Da (predicted); Q14114-5: Da (predicted).
O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain (By similarity);Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation (By similarity);Tyrosine phosphorylated upon apoE binding;Ubiquitinated by MYLIP leading to degradation -
Subunit
Homooligomer (PubMed:30873003). Interacts with VLDLR (PubMed:30873003). Reelin associates with two or more receptor molecules (PubMed:20223215). Interacts with DAB1 and JNK-interacting proteins. Interacts with SNX17 (By similarity). Interacts with PCSK9. Interacts with MDK; this interaction is calcium dependent (By similarity). Interacts with CLU (PubMed:24381170)
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SwissProt ID
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Synonyms
LRP8; APOER2; Low-density lipoprotein receptor-related protein 8; LRP-8; Apolipoprotein E receptor 2
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Research Field
Neuroscience
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