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PMP70 Antibody (YA1628)

(Synonyms: ABCD3; ABC43; PMP70; PXMP1; ZWS2)
Customer Review

Based on 1 Customer Validation

PMP70 Antibody (YA1628) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMP70.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • PMP70 Antibody (YA1628) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

PMP70 Antibody (YA1628) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMP70.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PMP70 aa620-659.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103980

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PMP70 Antibody (YA1628)
    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Mouse liver (lane 2, 20 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), SH-SY5Y (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PMP70 is a Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation. Has fatty acyl-CoA thioesterase and ATPase activities. Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes. Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation[1][2][3][4][5].

  • Subcellular Localization

    Peroxisome membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P28288 has 3 isomers: P28288-1: 75476 Da (predicted); P28288-2: 62726 Da (predicted); P28288-3: 27121 Da (predicted).
    Ubiquitinated by PEX2 during pexophagy in response to starvation, leading to its degradation

  • Subunit

    Homodimers (PubMed:17609205). Can form heterodimers with ABCD1 and ABCD2 (PubMed:10551832, PubMed:10777694, PubMed:17609205). Dimerization is necessary to form an active transporter (PubMed:17609205). Interacts with PEX19; mediates the targeting of ABCD3 to peroxisomes (PubMed:10704444, PubMed:16344115, PubMed:17761678)

  • SwissProt ID

    P28288

  • Gene ID
    5825 [NCBI]
  • Synonyms

    ABCD3; ABC43; PMP70; PXMP1; ZWS2

  • Research Field

    Neuroscience

PMP70 Antibody (YA1628) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Mouse liver (lane 2, 20 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), SH-SY5Y (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PMP70 Antibody (YA1628) Product Image
    1/16
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • PMP70 Antibody (YA1628) Product Image
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Mouse liver (lane 2, 20 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), SH-SY5Y (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PMP70 Antibody (YA1628)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • Experimental Validation Results for PMP70 Antibody (YA1628)
  • PMP70 Antibody (YA1628) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), and Mouse liver (lane 2, 20 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PMP70 Antibody (YA1628) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), SH-SY5Y (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using PMP70 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PMP70 Antibody (YA1628) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PMP70 Antibody (YA1628) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PMP70 with PMP70 Antibody (HY-P81883) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PMP70 Antibody (HY-P81883) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PMP70 Antibody (YA1628) Product Image WB
  • PMP70 Antibody (YA1628) Product Image WB
  • PMP70 Antibody (YA1628) Product Image ICC
  • PMP70 Antibody (YA1628) Product Image ICC