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Cleaved-Caspase 3 p17 Antibody (YA4628)

(Synonyms: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1)
Customer Review

Based on 1 Customer Validation

Cleaved-Caspase 3 p17 Antibody (YA4628) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Cleaved-Caspase 3 p17 Antibody (YA4628) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 32, 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Immunogen

A synthesized peptide derived from human active Caspase-3 aa29-87/277.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719006

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Western blot analysis was performed on extracts from Jurkat (1µM Staurosporine,12 hours) (lane 1, 15 μg) using Cleaved-Caspase 3 p17 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    Immunohistochemical analysis of paraffin-embedded human triple negative breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    Cleaved-Caspase 3 p17 is a Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). Cleaves and inactivates interleukin-18 (IL18). Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:It is highly expressed in the lungs, spleen, heart, liver, and kidneys. It is moderately expressed in the brain and skeletal muscle, and lowly expressed in the testes. It is also present in many cell lines, with the highest expression in immune system cells.

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce

  • SwissProt ID

    P42574

  • Gene ID
    836 [NCBI]
  • Synonyms

    CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Cleaved-Caspase 3 p17 Antibody (YA4628) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (1µM Staurosporine,12 hours) (lane 1, 15 μg) using Cleaved-Caspase 3 p17 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human triple negative breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    1/16
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (1µM Staurosporine,12 hours) (lane 1, 15 μg) using Cleaved-Caspase 3 p17 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human triple negative breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Experimental Validation Results for Cleaved-Caspase 3 p17 Antibody (YA4628)
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (1µM Staurosporine,12 hours) (lane 1, 15 μg) using Cleaved-Caspase 3 p17 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human triple negative breast cancer tissue using Cleaved-Caspase 3 Antibody (YA4628). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P84931, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image WB
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image IHC-P
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image IHC-P
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image IHC-P
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image IHC-P
  • Cleaved-Caspase 3 p17 Antibody (YA4628) Product Image IHC-P