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IL-1 alpha Antibody

(Synonyms: interleukin-1 alpha; Hematopoietin 1; IL 1 alpha; IL1 alpha; IL 1; IL 1A; Il-1a; IL1; IL1A; IL1F1; ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; Preinterleukin 1 alpha; Pro interleukin 1 alpha; Prointerleukin 1 alpha; IL1A_HUMAN. )
Customer Review

Based on 1 Customer Validation

IL-1 alpha Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, FC, IF-Tissue

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC FC
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • IL-1 alpha Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1ug/Test 1:100-500

Product Details

Description

IL-1 alpha Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 alpha.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse Predicted Reactivity: Rat
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from rat IL-1 Alpha: 155-230/270

Sensitivity

Endogenous

Purification

affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103065

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-1 alpha Antibody
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    Immunohistochemical analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human skin cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    Flow cytometric analysis of 1X106 Jurkat cells labeling IL-1 alpha Antibody (HY-P81115, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1ug:Test for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    IL-1 alpha is a major agonist of IL-1 and also is a pro-inflammatory cytokine. IL-1 alpha acts as an ‘alarmin’ and as a primum movens of tissue inflammation[1]. IL-1 alpha inhibits differentiation of preadipocytes and lipid accumulation[2]. IL-1 alpha induces apoptosis and inhibits the osteoblast differentiation[3]. IL-1 alpha trigger inflammation in a pathway initiated through Myd88 activation and culminated in NF-κB–induced transcription of inflammatory genes[4].

  • Subcellular Localization

    Nucleus; Cytoplasm; Secreted

  • Subunit

    Monomer. Interacts with TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059). Interacts with IL1R1 (PubMed:2950091). Interacts with S100A13; this interaction is the first step in the export of IL1A, followed by direct translocation of this complex across the plasma membrane (PubMed:21270123)

  • SwissProt ID

    P01583

  • Gene ID
    3552 [NCBI]
  • Synonyms

    interleukin-1 alpha; Hematopoietin 1; IL 1 alpha; IL1 alpha; IL 1; IL 1A; Il-1a; IL1; IL1A; IL1F1; ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; Preinterleukin 1 alpha; Pro interleukin 1 alpha; Prointerleukin 1 alpha; IL1A_HUMAN.

[1]. Schafer S, et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature. 2017 Dec 7;552(7683):110-115.

[2]. Cook SA, et al. Hiding in Plain Sight: Interleukin-11 Emerges as a Master Regulator of Fibrosis, Tissue Integrity, and Stromal Inflammation. Annu Rev Med. 2020 Jan 27;71:263-276. [Content Brief]

[3]. Balakrishnan L, et al. IL-11/IL11RA receptor mediated signaling: a web accessible knowledgebase. Cell Commun Adhes. 2013 Aug;20(3-4):81-6 [Content Brief]

[4]. Dams-Kozlowska H, et al. A designer hyper interleukin 11 (H11) is a biologically active cytokine. BMC Biotechnol. 2012 Mar 21;12:8. [Content Brief]

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IL-1 alpha Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human skin cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling IL-1 alpha Antibody (HY-P81115, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1ug:Test for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IL-1 alpha Antibody Product Image
    1/16
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • IL-1 alpha Antibody Product Image
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human skin cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for IL-1 alpha Antibody
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling IL-1 alpha Antibody (HY-P81115, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1ug:Test for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • Experimental Validation Results for IL-1 alpha Antibody
  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • IL-1 alpha Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • IL-1 alpha Antibody Product Image
    MedChemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • IL-1 alpha Antibody Product Image
    MedChemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human skin cancer tissue using IL-1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81115, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • IL-1 alpha Antibody Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling IL-1 alpha Antibody (HY-P81115, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1ug:Test for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IL-1 alpha Antibody Product Image IHC-P
  • IL-1 alpha Antibody Product Image IHC-P
  • IL-1 alpha Antibody Product Image IHC-P
  • IL-1 alpha Antibody Product Image mIHC
  • IL-1 alpha Antibody Product Image mIHC
  • IL-1 alpha Antibody Product Image mIHC
  • IL-1 alpha Antibody Product Image mIHC
  • IL-1 alpha Antibody Product Image mIHC
  • IL-1 alpha Antibody Product Image FC