p38 MAPK Antibody (YA8861)
(Synonyms: CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, MAPK14, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, Stress-activated protein kinase 2a, CSAID-binding protein, MAP kinase p38 alpha, SAPK2a)
Based on 1 Customer Validation
p38 MAPK Antibody (YA8861) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p38 MAPK. p38 MAPK Antibody detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:100-1:200 | 1:100-1:200 |
Product Details
p38 MAPK Antibody (YA8861) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p38 MAPK. p38 MAPK Antibody detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 41 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41 kDa
Entrez Gene: 1432 Human ; 26416 Mouse ;
SwissProt: Q16539 Human ; Q15759 Human ; P53778 Human ; O15264 Human ; P47811 Mouse ; Q9WUI1 Mouse ; O08911 Mouse ; Q9Z1B7 Mouse ; P70618 Rat ; D4A3U7 Rat ; Q63538 Rat ; Q9WTY9 Rat
OMIM: 600289 Human
A synthetic peptide of human p38
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
The p38 MAPK family consists of highly conserved proline-directed serine-threonine protein kinases that are activated in response to a number many growth factors, cytokines, and chemotactic substances, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), PDGF, TNF, interleukins, lipopolysaccharide (LPS) and formyl-methionyl-leucyl-phenylalanine (fMLP) . It is well known that p38 is involved in inflammation, apoptosis, cardiomyocyte hypertrophy and cell differentiation. The p38 MAPK family is composed of four proteins: p38α (encoded by the gene Mapk14), p38β (Mapk11), p38γ (Mapk12), and p38δ (Mapk13) . Their coding genes have a distinct tissue distribution and they appear differentially expressed, being Mapk14 the most highly expressed. p38 MAPKs are substrates for three MAP2K (MKK6, MKK3, and MKK4) . The contribution of each of these MAP2K to p38 MAPKs activation depends on the stimulus and the cell type. The MAP3Ks that lead to p38 MAPKs activation are ASK1, DLK1, TAK1, TAO1, TAO2, TPL2, MLK3, MEKK3, MEKK4, and ZAK1.
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:It is expressed at higher levels in the brain, heart, placenta, pancreas, and skeletal muscle. It is expressed at lower levels in the lungs, liver, and kidneys. -
Isoforms & Post-Translational Modification
Q16539 has 5 isomers: Q16539-1: 41293 Da (predicted); Q16539-2: 41493 Da (predicted); Q16539-3: 34092 Da (predicted); Q16539-4: 35453 Da (predicted); Q16539-5: 29388 Da (predicted).
Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 (PubMed:21283629);Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3;Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway -
Subunit
Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381). Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Interacts with PPM1D. Interacts with CDK5RAP3; recruits PPM1D to MAPK14 and may regulate its dephosphorylation (PubMed:21283629). Interacts with DUSP2; this interaction does not lead to catalytic activation of DUSP2 and dephosphrylation of MAPK14 (By similarity)
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SwissProt ID
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Synonyms
CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, MAPK14, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, Stress-activated protein kinase 2a, CSAID-binding protein, MAP kinase p38 alpha, SAPK2a
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Research Field
Signal Transduction
Documentation
References
[2]. Ganguly P, et al. Revisiting p38 Mitogen-Activated Protein Kinases (MAPK) in Inflammatory Arthritis: A Narrative of the Emergence of MAPK-Activated Protein Kinase Inhibitors (MK2i). Pharmaceuticals (Basel). 2023 Sep 12;16(9):1286. [Content Brief]
[3]. Carlini MJ, et al. Identification of p38 MAPK inhibition as a neuroprotective strategy for combinatorial SMA therapy. EMBO Mol Med. 2025 Oct;17(10):2762-2786. [Content Brief]
[4]. Falcicchia C, et al. Involvement of p38 MAPK in Synaptic Function and Dysfunction. Int J Mol Sci. 2020 Aug 6;21(16):5624. [Content Brief]
[6]. Corre I, et al. The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells. Oncotarget. 2017 May 29;8(33):55684-55714. [Content Brief]
[7]. Jiang R, et al. Effectiveness of exercise on perinatal depression and anxiety symptoms: A network meta-analysis and dose-response analysis. Int J Gynaecol Obstet. 2026 Jun;173(3):1308-1324. [Content Brief]
[8]. Onishi T, et al. The PERK-p38 MAPK Axis Drives Endoplasmic Reticulum Stress-Induced Apoptosis in Fuchs Endothelial Corneal Dystrophy. Invest Ophthalmol Vis Sci. 2025 Aug 1;66(11):63. [Content Brief]
[9]. Sun Y, et al. Suppression of Alzheimer's disease-related phenotypes by the heat shock protein 70 inducer, geranylgeranylacetone, in APP/PS1 transgenic mice via the ERK/p38 MAPK signaling pathway. Exp Ther Med. 2017 Dec;14(6):5267-5274. [Content Brief]
[10]. Hermans A, et al. A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury. Zebrafish. 2024 Apr;21(2):144-148. [Content Brief]
[11]. Chen S, et al. Activation of melanocortin receptor 4 with RO27-3225 attenuates neuroinflammation through AMPK/JNK/p38 MAPK pathway after intracerebral hemorrhage in mice. J Neuroinflammation. 2018 Apr 11;15(1):106. [Content Brief]
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