Copy Link Print Information

CYP7B1 Antibody (YA7801)

(Synonyms: CBAS3; CP7B; SPG5A)
Customer Review

Based on 1 Customer Validation

CYP7B1 Antibody (YA7801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP7B1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • CYP7B1 Antibody (YA7801) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-2000 1:50-5000

Product Details

Description

CYP7B1 Antibody (YA7801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP7B1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 58.1 kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 263-506 of human CYP7B1 produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Western blot analysis of extracts from HT-1080(lane 2, 20μg), HepG2(lane 3, 20μg), HeLa(lane 4, 20μg) and PC-3(lane 5, 20μg) using CYP7B1 Antibody (HY-P88117). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CYP7B1 is an A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives[1][2][3].

  • Subcellular Localization

    Endoplasmic reticulum membrane,Microsome membrane

  • Expression


    Tissue_Specificity: Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen

  • Isoforms & Post-Translational Modification

    O75881: 506 amino acids, molecular weight 58256 Da.

  • SwissProt ID

    O75881

  • Synonyms

    CBAS3; CP7B; SPG5A

CYP7B1 Antibody (YA7801) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-1080(lane 2, 20μg), HepG2(lane 3, 20μg), HeLa(lane 4, 20μg) and PC-3(lane 5, 20μg) using CYP7B1 Antibody (HY-P88117). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    1/16
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • CYP7B1 Antibody (YA7801) Product Image
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-1080(lane 2, 20μg), HepG2(lane 3, 20μg), HeLa(lane 4, 20μg) and PC-3(lane 5, 20μg) using CYP7B1 Antibody (HY-P88117). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • Experimental Validation Results for CYP7B1 Antibody (YA7801)
  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-1080(lane 2, 20μg), HepG2(lane 3, 20μg), HeLa(lane 4, 20μg) and PC-3(lane 5, 20μg) using CYP7B1 Antibody (HY-P88117). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human glioma tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using CYP7B1 Antibody (HY-P88117, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CYP7B1 Antibody (YA7801) Product Image WB
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P
  • CYP7B1 Antibody (YA7801) Product Image IHC-P