PDE4D Antibody (YA1714)

Art. -Nr.: HY-P81969: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

PDE4D Antibody (YA1714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4D.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe	Verfügbarkeit	Menge
Kostenlose Probe	Apply now
10 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

or Massenanfrage

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

PDE4D Antibody (YA1714) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

PDE4D Antibody (YA1714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4D.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molekulargewicht

Predicted band size: 91 kDa;

Observed band size: 91 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q08499

Gene ID

5144 [NCBI]

Immunogen

A synthesized peptide derived from human PDE4D aa650-809/809.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Reinheit	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using PDE4D (HY-P81969) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes

Subcellular Localization：Apical cell membrane; Cytoplasm; Membrane; Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_specificity:Expressed in colonic epithelial cells (at protein level) . Widespread; most abundant in skeletal muscle; Detected in brain; Detected in brain, placenta, lung and kidney; Detected in heart and skeletal muscle

Isoforms & Post-Translational Modification：Q08499 has 12 isomers: Q08499-1: 91115 Da (predicted); Q08499-2: 76467 Da (predicted); Q08499-3: 68607 Da (predicted); Q08499-4: 66376 Da (predicted); Q08499-5: 57792 Da (predicted); Q08499-6: 84428 Da (predicted); Q08499-7: 23839 Da (predicted); Q08499-8: 59113 Da (predicted); Q08499-9: 77705 Da (predicted); Q08499-10: 76816 Da (predicted); Q08499-11: 84662 Da (predicted); Q08499-12: 24429 Da (predicted).
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59;Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation

Subunit：Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner

RRID

AB_3104090

Database

Entrez Gene: 5144 Human

SwissProt: Q08499 Human

OMIM: 614613 Human

Research Field

Signal Transduction

Synonyms

cAMP-specific 3' ; 5' -cyclic phosphodiesterase 4D; DPDE3; PDE43; PDE4D; DPDE3

Dokumentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PDE4D Antibody (YA1714) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Ihre kürzlich angesehenen Produkte:

Produktname

Requested Quantity *

Name des Antragstellers *

Anrede

E-Mail-Addresse *

Telefonnummer *

Department

Name der Organisation *

City

Bundesland

Country or Region *

Bemerkungen

Produktname

Lot #

Name des Antragstellers *

Country or Region *

E-Mail-Addresse *

Bemerkungen

Name
Email *

	Sorry, but the email address you supplied was invalid.

PDE4D Antibody (YA1714)

PDE4D Antibody (YA1714) Spezialitätsempfehlungen:

Product Detail

Verification Image

Background

Beschreibung

PDE4D Antibody (YA1714) Related Classifications

Ihre kürzlich angesehenen Produkte:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report