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PDE4D Antibody (YA1714)

(Synonyms: cAMP-specific 3' ; 5' -cyclic phosphodiesterase 4D; DPDE3; PDE43; PDE4D; DPDE3)
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Based on 1 Customer Validation

PDE4D Antibody (YA1714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4D.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PDE4D Antibody (YA1714)
  • PDE4D Antibody (YA1714) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PDE4D Antibody (YA1714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4D.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database

Entrez Gene: 5144 Human

SwissProt: Q08499 Human

Immunogen

A synthesized peptide derived from human PDE4D aa650-809/809.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104090

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDE4D Antibody (YA1714)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using PDE4D (HY-P81969) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PDE4D hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes

  • Subcellular Localization

    Apical cell membrane; Cytoplasm; Membrane; Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Tissue_specificity:Expressed in colonic epithelial cells (at protein level) . Widespread; most abundant in skeletal muscle; Detected in brain; Detected in brain, placenta, lung and kidney; Detected in heart and skeletal muscle

  • Isoforms & Post-Translational Modification

    Q08499 has 12 isomers: Q08499-1: 91115 Da (predicted); Q08499-2: 76467 Da (predicted); Q08499-3: 68607 Da (predicted); Q08499-4: 66376 Da (predicted); Q08499-5: 57792 Da (predicted); Q08499-6: 84428 Da (predicted); Q08499-7: 23839 Da (predicted); Q08499-8: 59113 Da (predicted); Q08499-9: 77705 Da (predicted); Q08499-10: 76816 Da (predicted); Q08499-11: 84662 Da (predicted); Q08499-12: 24429 Da (predicted).
    Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59;Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation

  • Subunit

    Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner

  • SwissProt ID

    Q08499

  • Gene ID
    5144 [NCBI]
  • Synonyms

    cAMP-specific 3' ; 5' -cyclic phosphodiesterase 4D; DPDE3; PDE43; PDE4D; DPDE3

  • Research Field

    Signal Transduction

PDE4D Antibody (YA1714) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using PDE4D (HY-P81969) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PDE4D Antibody (YA1714) Product Image
    1/16
  • Experimental Validation Results for PDE4D Antibody (YA1714)
  • PDE4D Antibody (YA1714) Product Image
  • Experimental Validation Results for PDE4D Antibody (YA1714)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using PDE4D (HY-P81969) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDE4D Antibody (YA1714)
  • PDE4D Antibody (YA1714) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using PDE4D (HY-P81969) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PDE4D Antibody (YA1714) Product Image WB