Phospho-KIF2C (Ser95) Antibody
(Synonyms: KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK)Phospho-KIF2C (Ser95) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-KIF2C (Ser95).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Monkey
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
Phospho-KIF2C (Ser95) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-KIF2C (Ser95).
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Monkey
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Observed Molecular WeightObserved band size: 81 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 81 kDa
Synthetic phosphopeptide corresponding to residues surrounding S95 of human KIF2C protein.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
KIF2C is an In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment[1][2][3].
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Subcellular Localization
Cytoplasm, cytoskeleton; Nucleus; Chromosome, centromere; Chromosome, centromere, kinetochore
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Expression
Tissue_Specificity: Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
Isoforms & Post-Translational Modification
Phospho-KIF2C has 2 isoforms, Q99661-1: amino acid length is 725, molecular weight is 81313 Da (predicted); Q99661-2: amino acid length is 671, molecular weight is 75561 Da (predicted).
Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
Subunit
Interacts with CENPH.
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SwissProt ID
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Synonyms
KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK
Documentation
[1]. Tanenbaum ME, et al. A complex of Kif18b and MCAK promotes microtubule depolymerization and is negatively regulated by Aurora kinases. Curr Biol. 2011 Aug 23;21(16):1356-65. [Content Brief]
[2]. Bakhoum SF, et al. Genome stability is ensured by temporal control of kinetochore-microtubule dynamics. Nat Cell Biol. 2009 Jan;11(1):27-35. [Content Brief]
[3]. Shrestha RL, et al. Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK. Curr Biol. 2013 Aug 19;23(16):1514-26. [Content Brief]
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