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Proteasome 20S LMP2 Antibody (YA1747)

(Synonyms: Beta1i; LMP2; PSMB 9; PSMB6i; PSMB9; RING12)
Customer Review

Based on 1 Customer Validation

Proteasome 20S LMP2 Antibody (YA1747) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Proteasome 20S LMP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Proteasome 20S LMP2 Antibody (YA1747) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Proteasome 20S LMP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Proteasome 20S LMP2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104123

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), HL-60 (lane 3, 15 μg), A431 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using PSMB9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Proteasome 20S LMP2 is a The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues[1][2].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Induction:Up-regulated by interferon gamma (at protein level) . Up-regulated by IRF1. Up-regulated by tumor necrosis factor-alpha (at protein level) . Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD) . Up-regulated by heat shock treatment. Up-regulated by CD40L via the NFKB1 pathway in cancer cells

  • Isoforms & Post-Translational Modification

    P28065 has 2 isomers: P28065-1: 23264 Da (predicted); P28065-2: 22328 Da (predicted).
    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity

  • Subunit

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis

  • SwissProt ID

    P28065

  • Gene ID
    5698 [NCBI]
  • Synonyms

    Beta1i; LMP2; PSMB 9; PSMB6i; PSMB9; RING12

  • Research Field

    Cell Biology

References

[1]. Kataoka S, et al. Successful treatment of a novel type I interferonopathy due to a de novo PSMB9 gene mutation with a Janus kinase inhibitor. J Allergy Clin Immunol. 2021 Aug;148(2):639-644. [Content Brief]

[2]. Kanazawa N, et al. Heterozygous missense variant of the proteasome subunit β-type 9 causes neonatal-onset autoinflammation and immunodeficiency. Nat Commun. 2021 Nov 24;12(1):6819. [Content Brief]

Proteasome 20S LMP2 Antibody (YA1747) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), HL-60 (lane 3, 15 μg), A431 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using PSMB9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
    1/16
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), HL-60 (lane 3, 15 μg), A431 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using PSMB9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Experimental Validation Results for Proteasome 20S LMP2 Antibody (YA1747)
  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), HL-60 (lane 3, 15 μg), A431 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using PSMB9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Proteasome 20S LMP2 Antibody (YA1747) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP2 with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Proteasome 20S LMP2 Antibody (HY-P82002) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Proteasome 20S LMP2 Antibody (YA1747) Product Image WB
  • Proteasome 20S LMP2 Antibody (YA1747) Product Image ICC
  • Proteasome 20S LMP2 Antibody (YA1747) Product Image ICC