CD19 Antibody (YA6339)
(Synonyms: CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12)CD19 Antibody (YA6339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD19.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
CD19 Antibody (YA6339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD19.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 110 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD19 is a B cell-specific transmembrane glycoprotein and a core member of the B cell receptor (BCR) co-receptor complex. It can significantly enhance BCR signaling by activating signaling pathways such as PI3K and BTK, promoting B cell development, activation, and antibody production. CD19 is present throughout almost the entire B cell development process and is a biomarker for all B cells. CD19 is an important marker for immunophenotyping of B-cell leukemia and lymphoma, and is also one of the targets for CAR-T cell therapy, bispecific antibodies, and other B cell-targeted therapies.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein
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Expression
Tissue_specificity:Detected in B cells in the marginal zone of lymph nodes and germinal centers (PubMed:2463100) . Detected in blood B cells (protein levels) (PubMed:16672701, PubMed:2463100) . -
Isoforms & Post-Translational Modification
CD19 has 2 isomers: P15391-1: 61128 Da (predicted); P15391-2: 61200 Da (predicted).
Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. -
Subunit
Interacts with CR2/CD21 (PubMed:1702139). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells (PubMed:1383329). Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells (PubMed:16449649). Interacts with VAV (PubMed:10706702). Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409 (PubMed:10706702). Interacts with LYN (PubMed:7687428). Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2) (PubMed:7684160). Interacts with GRB2 (PubMed:29523808)
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SwissProt ID
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Synonyms
CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12
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