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CCL3/MIP-1 alpha Antibody (YA6494)

(Synonyms: C C motif chemokine 3 antibody; CCL 3 antibody; CCL3 antibody; CCL3_HUMAN antibody; Chemokine (C C motif) ligand 3 antibody; Chemokine C C motif ligand 3 antibody; Chemokine ligand 3 antibody; G0/G1 switch regulatory protein 19 1 antibody; G0/G1 switch regulatory protein 19-1 antibody; G0S19 1 antibody; C C motif chemokine 3 antibody; CCL 3 antibody; CCL3 antibody; CCL3_HUMAN antibody; Chemokine (C C motif) ligand 3 antibody; Chemokine C C motif ligand 3 antibody; Chemokine ligand 3 antibody; G0/G1 switch regulatory protein 19 1 antibody; G0/G1 switch regulatory protein 19-1 antibody; G0S19 1 antibody; G0S19 1 protein antibody; Heparin binding chemotaxis protein antibody; L2G25B antibody; LD78 alpha antibody; LD78-alpha(4-69) antibody; LD78alpha antibody; Macrophage inflammatory protein 1 alpha antibody; Macrophage inflammatory protein 1-alpha antibody; macrophage inflammatory protein 1a antibody; M; IP : 1 alpha antibody; M; IP : 1A antibody; MIP-1-alpha antibody; MIP-1-alpha(4-69) antibody; MIP1 alpha antibody; MIP1A antibody; PAT 464.1 antibody; SCYA 3 antibody; SCYA3 antibody; SIS alpha antibody; SIS beta antibody; SIS-beta antibody; Small inducible cytokine A3 antibody; small inducible cytokine A3 (homologous to mouse Mip-1a) antibody; Small-inducible cytokine A3 antibody; Tonsillar lymphocyte LD78 alpha protein antibody; )
Customer Review

Based on 1 Customer Validation

CCL3/MIP-1 alpha Antibody (YA6494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCL3/MIP-1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:2000 1:200

Product Details

Description

CCL3/MIP-1 alpha Antibody (YA6494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCL3/MIP-1 alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 10 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 10 kDa
Immunogen

Recombinant protein within human CCL3 aa 1-92.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719214

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
    Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
    Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CCL3/MIP-1 alpha is a Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV)

  • Subcellular Localization

    Secreted

  • Expression

    Induction: By TPA or PHA (TPA = 12-O-tetradecanoyl phorbol-13 acetate (tumor promoter); PHA = phytohemagglutinin (T-cell mitogen))

  • Isoforms & Post-Translational Modification

    P10147: 92 amino acids, molecular weight 10085 Da.

  • Subunit

    Self-associates

  • SwissProt ID

    P10147

  • Gene ID
    6348 [NCBI]
  • Synonyms

    C C motif chemokine 3 antibody; CCL 3 antibody; CCL3 antibody; CCL3_HUMAN antibody; Chemokine (C C motif) ligand 3 antibody; Chemokine C C motif ligand 3 antibody; Chemokine ligand 3 antibody; G0/G1 switch regulatory protein 19 1 antibody; G0/G1 switch regulatory protein 19-1 antibody; G0S19 1 antibody; C C motif chemokine 3 antibody; CCL 3 antibody; CCL3 antibody; CCL3_HUMAN antibody; Chemokine (C C motif) ligand 3 antibody; Chemokine C C motif ligand 3 antibody; Chemokine ligand 3 antibody; G0/G1 switch regulatory protein 19 1 antibody; G0/G1 switch regulatory protein 19-1 antibody; G0S19 1 antibody; G0S19 1 protein antibody; Heparin binding chemotaxis protein antibody; L2G25B antibody; LD78 alpha antibody; LD78-alpha(4-69) antibody; LD78alpha antibody; Macrophage inflammatory protein 1 alpha antibody; Macrophage inflammatory protein 1-alpha antibody; macrophage inflammatory protein 1a antibody; M; IP : 1 alpha antibody; M; IP : 1A antibody; MIP-1-alpha antibody; MIP-1-alpha(4-69) antibody; MIP1 alpha antibody; MIP1A antibody; PAT 464.1 antibody; SCYA 3 antibody; SCYA3 antibody; SIS alpha antibody; SIS beta antibody; SIS-beta antibody; Small inducible cytokine A3 antibody; small inducible cytokine A3 (homologous to mouse Mip-1a) antibody; Small-inducible cytokine A3 antibody; Tonsillar lymphocyte LD78 alpha protein antibody;

CCL3/MIP-1 alpha Antibody (YA6494) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image
    1/16
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • Experimental Validation Results for CCL3/MIP-1 alpha Antibody (YA6494)
  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:1000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling CCL3/MIP-1 alpha with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CCL3/MIP-1 alpha Antibody (HY-P86801) at 1:2000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image ICC
  • CCL3/MIP-1 alpha Antibody (YA6494) Product Image ICC