FoxD3 Antibody (YA4568)

Art. -Nr.: HY-P84871: Data Sheet COA
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User Guide for Antibodies
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FoxD3 Antibody (YA4568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FoxD3.

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

FoxD3 Antibody (YA4568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FoxD3.

Host

Mouse

Clonality

Monoclonal Antibody

Molekulargewicht

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

Q9UJU5

Gene ID

27022 [NCBI]

Immunogen

Purified recombinant fragment of human FOXD3 aa 132-293.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(40μg),JuRKAT(lane 3(40μg), K562(lane 4(40μg),RAW264.7(lane 5(40μg)using FoxD3 Antibody ( Mouse). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room te

Background

Function：Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor (PubMed:11891324). Also acts as a transcriptional activator (PubMed:11891324). Negatively regulates transcription of transcriptional repressor RHIT/ZNF205 (PubMed:22306510). Promotes development of neural crest cells from neural tube progenitors (PubMed:11891324). Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation (PubMed:11891324). Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis (PubMed:11891324)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:This gene is expressed in chronic myeloid leukemia, Jurkat T-cell leukemia, and teratoma cell lines, but not in other cell lines or normal tissues tested.

Subunit：Interacts with POU5F1

RRID

AB_3718993

Synonyms

AIS1; HFH2; Genesis; FOXD3

Dokumentation

Select Batch:

Data Sheet (259 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)