FoxD3 Antibody (YA4568)
(Synonyms: AIS1; HFH2; Genesis; FOXD3)Based on 1 Customer Validation
FoxD3 Antibody (YA4568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FoxD3.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ELISA, ELISA
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Reactivity :
Human, Mouse, Monkey
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:10000 |
Product Details
FoxD3 Antibody (YA4568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FoxD3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Monkey
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Observed Molecular WeightObserved band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 48 kDa
Purified recombinant fragment of human FOXD3 aa 132-293.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela(lane 2(40μg),JuRKAT(lane 3(40μg), K562(lane 4(40μg),RAW264.7(lane 5(40μg)using FoxD3 Antibody ( Mouse). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room te
Background
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Function
FoxD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Negatively regulates transcription of transcriptional repressor RHIT/ZNF205. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:This gene is expressed in chronic myeloid leukemia, Jurkat T-cell leukemia, and teratoma cell lines, but not in other cell lines or normal tissues tested. -
Subunit
Interacts with POU5F1
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SwissProt ID
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Synonyms
AIS1; HFH2; Genesis; FOXD3
Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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