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Epac2 Antibody (YA2323)

(Synonyms: CAMP GEFII; CAMPGEFII; CGEF2; EPAC2; Rapgef4)
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Based on 1 Customer Validation

Epac2 Antibody (YA2323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Epac2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Epac2 Antibody (YA2323)
  • Epac2 Antibody (YA2323) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Epac2 Antibody (YA2323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Epac2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 116 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 116 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Epac2 aa900-1011/1011.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104679

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Epac2 Antibody (YA2323)
    Western blot analysis of extracts from HL-60 (lane 2(20μg), HL-60 (lane 3(40μg), using Epac2 Antibody (YA2323). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Epac2 is a Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2

  • Subcellular Localization

    Cytoplasm; Membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels

  • Isoforms & Post-Translational Modification

    Q8WZA2 has 5 isomers: Q8WZA2-1: 115522 Da (predicted); Q8WZA2-2: 79492 Da (predicted); Q8WZA2-3: 99523 Da (predicted); Q8WZA2-4: 98248 Da (predicted); Q8WZA2-5: 96269 Da (predicted).

  • Subunit

    Interacts with RIMS1 and RIMS2.

  • SwissProt ID

    Q8WZA2

  • Gene ID
    11069 [NCBI]
  • Synonyms

    CAMP GEFII; CAMPGEFII; CGEF2; EPAC2; Rapgef4

  • Research Field

    Signal Transduction

Epac2 Antibody (YA2323) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL-60 (lane 2(20μg), HL-60 (lane 3(40μg), using Epac2 Antibody (YA2323). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Epac2 Antibody (YA2323) Product Image
    1/16
  • Experimental Validation Results for Epac2 Antibody (YA2323)
  • Epac2 Antibody (YA2323) Product Image
  • Experimental Validation Results for Epac2 Antibody (YA2323)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL-60 (lane 2(20μg), HL-60 (lane 3(40μg), using Epac2 Antibody (YA2323). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Epac2 Antibody (YA2323)
  • Epac2 Antibody (YA2323) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL-60 (lane 2(20μg), HL-60 (lane 3(40μg), using Epac2 Antibody (YA2323). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Epac2 Antibody (YA2323) Product Image WB