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Menin Antibody (YA1673)

(Synonyms: MEN1; SCG2; Menin)
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Based on 1 Customer Validation

Menin Antibody (YA1673) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Menin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Menin Antibody (YA1673)
  • Menin Antibody (YA1673) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:100-1:500 1:20-1:50 1:10-100

Product Details

Description

Menin Antibody (YA1673) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Menin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Menin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Menin Antibody (YA1673)
    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), C6 (lane 3, 20ug), HCT116 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using Menin Antibody (HY-P81928). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Menin is essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    O00255 has 3 isomers: O00255-2: 67497 Da (predicted); O00255-1: 68023 Da (predicted); O00255-3: 63647 Da (predicted).

  • Subunit

    Component of the MLL-HCF complex, at least composed of KMT2A/MLL1, MEN1, ASH2L, RBBP5, DPY30, WDR5, HCFC1 and HCFC2. Component of the menin-associated histone methyltransferase complex, at least composed of KMT2B/MLL4, MEN1, ASH2L, RBBP5, DPY30 and WDR5. Interacts with POLR2B. Interacts with POLR2A phosphorylated at 'Ser-5', but not with the unphosphorylated, nor 'Ser-2' phosphorylated POLR2A forms. Interacts with FANCD2 and DBF4. Interacts with JUND (via MBM motif); inhibits the interaction of JUND with MAPK10 and the phosphorylation of JUND by MAP kinases MAPK8 and MAPK10 (PubMed:22327296, PubMed:9989505). Interacts with SMAD3, but not with SMAD2, nor SMAD4. Directly interacts with NFKB1, NFKB2 and RELA. Interacts with KMT2A (via MBM motif) (PubMed:22327296, PubMed:22936661, PubMed:25305204). The KMT2A-MEN1 complex interacts with PSIP1 with a greater affinity as MEN1 enhances interaction of KMT2A with PSIP1 (PubMed:22327296, PubMed:25305204). Interacts with the fusion protein KMT2A-MLLT3 (PubMed:22936661, PubMed:25305204)

  • SwissProt ID

    O00255

  • Gene ID
    4221 [NCBI]
  • Synonyms

    MEN1; SCG2; Menin

  • Research Field

    Epigenetics and Nuclear Signaling

Menin Antibody (YA1673) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), C6 (lane 3, 20ug), HCT116 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using Menin Antibody (HY-P81928). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Menin Antibody (YA1673) Product Image
    1/16
  • Experimental Validation Results for Menin Antibody (YA1673)
  • Menin Antibody (YA1673) Product Image
  • Experimental Validation Results for Menin Antibody (YA1673)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), C6 (lane 3, 20ug), HCT116 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using Menin Antibody (HY-P81928). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Menin Antibody (YA1673)
  • Menin Antibody (YA1673) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), C6 (lane 3, 20ug), HCT116 (lane 4, 20ug) and Jurkat (lane 5, 20ug) using Menin Antibody (HY-P81928). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Menin Antibody (YA1673) Product Image WB