Copy Link Print Information

PKLR Antibody (YA7843)

(Synonyms: PK1; PKL; PKR; PKRL; RPK)
Customer Review

Based on 1 Customer Validation

PKLR Antibody (YA7843) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PKLR.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • PKLR Antibody (YA7843) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000-3000 1:100-250 1:100

Product Details

Description

PKLR Antibody (YA7843) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PKLR.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 61.6 kDa
Immunogen

Full length human recombinant protein of human PKLR produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PKLR Antibody (YA7843)
    Western blot analysis of extracts from Jurkat(lane 2, 20μg), Raji(lane 3, 20μg), K562(lane 4, 20μg) and THP-1(lane 5, 20μg) using PKLR Antibody (HY-P88159). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKLR Antibody (YA7843)
    Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PKLR Antibody (YA7843)
    Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PKLR is a Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis

  • Isoforms & Post-Translational Modification

    P30613 has two isomers: P30613-1: 61830 Da (predicted); P30613-2: 58494 Da (predicted).

  • Subunit

    Homotetramer

  • SwissProt ID

    P30613

  • Synonyms

    PK1; PKL; PKR; PKRL; RPK

PKLR Antibody (YA7843) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2, 20μg), Raji(lane 3, 20μg), K562(lane 4, 20μg) and THP-1(lane 5, 20μg) using PKLR Antibody (HY-P88159). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PKLR Antibody (YA7843) Product Image
    1/16
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • PKLR Antibody (YA7843) Product Image
  • Experimental Validation Results for PKLR Antibody (YA7843)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2, 20μg), Raji(lane 3, 20μg), K562(lane 4, 20μg) and THP-1(lane 5, 20μg) using PKLR Antibody (HY-P88159). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKLR Antibody (YA7843)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PKLR Antibody (YA7843)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • Experimental Validation Results for PKLR Antibody (YA7843)
  • PKLR Antibody (YA7843) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2, 20μg), Raji(lane 3, 20μg), K562(lane 4, 20μg) and THP-1(lane 5, 20μg) using PKLR Antibody (HY-P88159). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • PKLR Antibody (YA7843) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PKLR Antibody (YA7843) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PKLR with PKLR Antibody (HY-P88159) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PKLR Antibody (HY-P88159) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PKLR Antibody (YA7843) Product Image WB
  • PKLR Antibody (YA7843) Product Image ICC
  • PKLR Antibody (YA7843) Product Image ICC