UFM1 Antibody (YA1577)(PBS only)

Cat. No.: HY-P81832A: Data Sheet User Guide for Antibodies
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UFM1 Antibody (YA1577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFM1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

UFM1 Antibody (YA1577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFM1.

Host

Rabbit

Molecular Weight

Predicted band size: 9 kDa;

Observed band size: 9 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P61960

Gene ID

51569 [NCBI]

Immunogen

A synthetic peptide of human UFM1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer (PubMed:15071506, PubMed:20018847, PubMed:27653677, PubMed:29868776, PubMed:30626644, PubMed:38377992, PubMed:38383785). The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847, PubMed:27653677, PubMed:29868776). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:25219498, PubMed:32160526, PubMed:38383785)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Induction:Up-regulated by thapsigargin

Isoforms & Post-Translational Modification：P61960 has 2 isomers: P61960-1: 9118 Da (predicted); P61960-2: 10960 Da (predicted).
UFM1 precursor is cleaved by UFSP1, promoting its maturation: processing of the C-terminal Ser-Cys dipeptide is required to expose its C-terminal conserved Gly residue

Subunit：Interacts with UBA5 (PubMed:26872069, PubMed:26929408, PubMed:27653677, PubMed:28360427, PubMed:29295865, PubMed:30412706). Interacts with UFC1 (PubMed:29868776)

Research Field

Cell Biology

Synonyms

Ubiquitin-fold modifier 1; C13orf20

Documentation

User Guide for Antibodies (1077 KB)