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UFM1 Antibody (YA1577)(PBS only)

(Synonyms: Ubiquitin-fold modifier 1; C13orf20)

UFM1 Antibody (YA1577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFM1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • UFM1 Antibody (YA1577)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

UFM1 Antibody (YA1577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFM1.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 9 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 9 kDa
Immunogen

A synthetic peptide of human UFM1

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , RAW264.7(lane 3(20ug) and Jurkat(lane 4(20ug) using UFM1 Antibody (HY-P81832) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    UFM1 is an Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Induction:Up-regulated by thapsigargin

  • Isoforms & Post-Translational Modification

    P61960 has 2 isomers: P61960-1: 9118 Da (predicted); P61960-2: 10960 Da (predicted).
    UFM1 precursor is cleaved by UFSP1, promoting its maturation: processing of the C-terminal Ser-Cys dipeptide is required to expose its C-terminal conserved Gly residue

  • Subunit

    Interacts with UBA5 (PubMed:26872069, PubMed:26929408, PubMed:27653677, PubMed:28360427, PubMed:29295865, PubMed:30412706). Interacts with UFC1 (PubMed:29868776)

  • SwissProt ID

    P61960

  • Gene ID
    51569 [NCBI]
  • Synonyms

    Ubiquitin-fold modifier 1; C13orf20

  • Research Field

    Cell Biology

UFM1 Antibody (YA1577)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , RAW264.7(lane 3(20ug) and Jurkat(lane 4(20ug) using UFM1 Antibody (HY-P81832) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • UFM1 Antibody (YA1577)(PBS only) Product Image
    1/16
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • UFM1 Antibody (YA1577)(PBS only) Product Image
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , RAW264.7(lane 3(20ug) and Jurkat(lane 4(20ug) using UFM1 Antibody (HY-P81832) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • Experimental Validation Results for UFM1 Antibody (YA1577)(PBS only)
  • UFM1 Antibody (YA1577)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , RAW264.7(lane 3(20ug) and Jurkat(lane 4(20ug) using UFM1 Antibody (HY-P81832) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • UFM1 Antibody (YA1577)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • UFM1 Antibody (YA1577)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using UFM1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • UFM1 Antibody (YA1577)(PBS only) Product Image WB
  • UFM1 Antibody (YA1577)(PBS only) Product Image IHC-P
  • UFM1 Antibody (YA1577)(PBS only) Product Image IHC-P