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MCM3 Antibody (YA3909)

(Synonyms: HCC5; P1.h; RLFB; P1-MCM3)
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Based on 1 Customer Validation

MCM3 Antibody (YA3909) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCM3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • MCM3 Antibody (YA3909) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:50-1:200 1:200-1:400

Product Details

Description

MCM3 Antibody (YA3909) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCM3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Immunogen

Purified recombinant fragment of human MCM3 aa 293-442.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718858

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MCM3 Antibody (YA3909)
    Western blot analysis of extracts from Raji (lane 2(20μg), HepG2 (lane 3(20μg), HEK293T (lane 4(20μg), SH-SY5Y (lane 5(20μg) using MCM3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MCM3 Antibody (YA3909)
    Flow cytometric analysis of 1X106 HeLa cells labeling MCM3 Antibody (HY-P84212, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MCM3 acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable)[1][2][3][4].

  • Subcellular Localization

    Nucleus; Chromosome

  • Isoforms & Post-Translational Modification

    P25205 has 2 isomers: P25205-1: 90981 Da (predicted); P25205-2: 95908 Da (predicted).
    Acetylated by MCM3AP;O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner

  • Subunit

    Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731). The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (PubMed:16899510, PubMed:17296731, PubMed:32453425, PubMed:34694004, PubMed:34700328).

  • SwissProt ID

    P25205

  • Gene ID
    4172 [NCBI]
  • Synonyms

    HCC5; P1.h; RLFB; P1-MCM3

MCM3 Antibody (YA3909) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), HepG2 (lane 3(20μg), HEK293T (lane 4(20μg), SH-SY5Y (lane 5(20μg) using MCM3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MCM3 Antibody (HY-P84212, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MCM3 Antibody (YA3909) Product Image
    1/16
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • MCM3 Antibody (YA3909) Product Image
  • Experimental Validation Results for MCM3 Antibody (YA3909)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), HepG2 (lane 3(20μg), HEK293T (lane 4(20μg), SH-SY5Y (lane 5(20μg) using MCM3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MCM3 Antibody (YA3909)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MCM3 Antibody (HY-P84212, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • Experimental Validation Results for MCM3 Antibody (YA3909)
  • MCM3 Antibody (YA3909) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji (lane 2(20μg), HepG2 (lane 3(20μg), HEK293T (lane 4(20μg), SH-SY5Y (lane 5(20μg) using MCM3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MCM3 Antibody (YA3909) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling MCM3 Antibody (HY-P84212, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MCM3 Antibody (YA3909) Product Image WB
  • MCM3 Antibody (YA3909) Product Image FC