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Integrin Linked ILK Antibody (YA3111)

(Synonyms: ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK)
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Based on 1 Customer Validation

Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Integrin Linked ILK Antibody (YA3111) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Integrin linked ILK

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105479

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and U2OS (lane 2, 15 μg) using Integrin Linked ILK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Integrin Linked ILK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Integrin Linked ILK is a Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes. Acts as an upstream effector of both AKT1/PKB and GSK3. Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA. Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus[1][2][3][4][5][6][7].

  • Subcellular Localization

    Cell junction, focal adhesion; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium; Cytoplasm, myofibril, sarcomere; Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cell cortex

  • Expression


    Tissue_specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver

  • Isoforms & Post-Translational Modification

    Q13418 has 3 isomers: Q13418-1: 51419 Da (predicted); Q13418-2: 44349 Da (predicted); Q13418-3: 36467 Da (predicted).
    Phosphorylation by PAK1 modulates ILK subcellular location by promoting its nuclear export

  • Subunit

    Component of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex composed of ILK, LIMS1/PINCH and PARVA; the complex binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling (PubMed:11331308, PubMed:12167643, PubMed:12432066, PubMed:19074270, PubMed:19117955, PubMed:21524996, PubMed:30367047, PubMed:35259013). Formation of the IPP complex is dependent on protein kinase C and precedes integrin-mediated cell adhesion and spreading (PubMed:12432066). ILK also interacts with LIMS2/PINCH2 and with PARVB and PARVG which may substitute for LIMS1 and PARVA in the IPP complex; PARVA and PARVB compete for the same binding site (PubMed:11402068, PubMed:12167643, PubMed:15284246, PubMed:15817463, PubMed:16517730, PubMed:20005845, PubMed:20117114). Interaction with PARVG promotes the establishment of cell polarity required for leukocyte migration (PubMed:16517730). Interacts with the cytoplasmic domain of integrin ITGB1 and may also interact with integrins ITGB2, ITGB3 and/or ITGB5 (PubMed:8538749). Interacts probably also with TGFB1I1 (By similarity). Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (PubMed:19118217). Interacts with FERMT2 (By similarity). Interacts with LIMD2; leading to activate the protein kinase activity (PubMed:24590809). Interacts with PXN/PAXILLIN (via LD motif 4) (PubMed:15817463). Interacts with CCDC25 (via cytoplasmic region); initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (PubMed:32528174). Interacts with IQGAP1; the interaction is required for localization of IQGAP1 to the cell cortex (By similarity)

  • SwissProt ID

    Q13418

  • Gene ID
    3611 [NCBI]
  • Synonyms

    ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK

  • Research Field

    Signal Transduction

Integrin Linked ILK Antibody (YA3111) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and U2OS (lane 2, 15 μg) using Integrin Linked ILK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Integrin Linked ILK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Integrin Linked ILK Antibody (YA3111) Product Image
    1/16
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Integrin Linked ILK Antibody (YA3111) Product Image
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and U2OS (lane 2, 15 μg) using Integrin Linked ILK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Integrin Linked ILK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Experimental Validation Results for Integrin Linked ILK Antibody (YA3111)
  • Integrin Linked ILK Antibody (YA3111) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and U2OS (lane 2, 15 μg) using Integrin Linked ILK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Integrin Linked ILK Antibody (YA3111) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Integrin Linked ILK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Integrin Linked ILK Antibody (YA3111) Product Image WB
  • Integrin Linked ILK Antibody (YA3111) Product Image WB